Costa Caio César Chaves, Pereira Nathalia Gabay, Machado Anna Luiza Melo, Dórea Mariana Albuquerque, Cruz Rafaella Macêdo Monteiro da, Silva Renata Cunha, Domingues Robson José de Souza, Yasojima Edson Yuzur
Graduate student, Faculty of Medicine, UEPA, Belem-PA, Brazil. Technical procedures, analysis and interpretation of data, manuscript preparation.
Fellow, Postgraduate Program in Surgery and Experimental Research, UEPA, Belem-PA, Brazil. Technical procedures, analysis and interpretation of data, manuscript preparation.
Acta Cir Bras. 2019 Sep 16;34(7):e201900707. doi: 10.1590/s0102-865020190070000007.
To evaluate the effects of splenic ischemic preconditioning (sIPC) on oxidative stress induced by hepatic ischemia-reperfusion in rats.
Fifteen male Wistar rats were equally divided into 3 groups: SHAM, IRI and sIPC. Animals from IRI group were subjected to 45 minutes of partial liver ischemia (70%). In the sIPC group, splenic artery was clamped in 2 cycles of 5 min of ischemia and 5 min of reperfusion (20 min total) prior to hepatic ischemia. SHAM group underwent the same surgical procedures as in the remaining groups, but no liver ischemia or sIPC were induced. After 1h, hepatic and splenic tissue samples were harvested for TBARS, CAT, GPx and GSH-Rd measurement.
sIPC treatment significantly decreased both hepatic and splenic levels of TBARS when compared to IRI group (p<0.01). Furthermore, the hepatic and splenic activities of CAT, GPx and GSH- Rd were significantly higher in sIPC group than in IRI group.
sIPC was able to attenuate hepatic and splenic IRI-induced oxidative stress.
评估脾脏缺血预处理（sIPC）对大鼠肝脏缺血再灌注诱导的氧化应激的影响。
将15只雄性Wistar大鼠平均分为3组：假手术组（SHAM）、缺血再灌注组（IRI）和脾脏缺血预处理组（sIPC）。IRI组动物接受45分钟的部分肝脏缺血（70%）。在sIPC组中，在肝脏缺血前，脾脏动脉进行2个周期的夹闭，每个周期缺血5分钟，再灌注5分钟（共20分钟）。SHAM组接受与其他组相同的手术操作，但不诱导肝脏缺血或sIPC。1小时后，采集肝脏和脾脏组织样本，用于测量丙二醛（TBARS）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GSH-Rd）。
与IRI组相比，sIPC治疗显著降低了肝脏和脾脏的TBARS水平（p<0.01）。此外，sIPC组肝脏和脾脏的CAT、GPx和GSH-Rd活性显著高于IRI组。
sIPC能够减轻肝脏和脾脏缺血再灌注诱导的氧化应激。
