Department of Urology, Northern Jiangsu People's Hospital, Yangzhou, China.
Eur Rev Med Pharmacol Sci. 2018 Apr;22(7):2134-2142. doi: 10.26355/eurrev_201804_14747.
To investigate the therapeutic effect of splenic ischemic preconditioning (sIPC) on renal ischemia-reperfusion (IR) injury.
A total of 18 adult male Sprague Dawley (SD) rats were treated by 45 min renal ischemia and followed by 24 h reperfusion. In the sIPC group, three cycles of splenic ischemic preconditioning including 5 min ischemia and 5 min reperfusion were carried out before renal ischemia. The blood samples and kidney tissues were collected after 24 h. The levels of Cr and BUN in serum were measured to evaluate the kidney function. The morphological changes in ischemia-reperfusion kidneys were determined by hematoxylin-eosin (HE) staining. The levels of pro-inflammatory cytokines including TNF-α and IL-6 in serum, and renal tissues, were measured by ELISA and Western Blotting. Furthermore, the levels of IKK-β, intra-nuclear NF-κB, p65, and IL-10 in renal tissues were also measured.
The results demonstrated that the level of Cr and BUN in the IR group were increased while decreased in the sIPC group. HE staining showed that the damage caused by renal ischemia-reperfusion was attenuated by sIPC with a low renal injury score in the sIPC group. ELISA and Western Blotting results showed that the production and secretion of TNF-α and IL-6 induced by IR were inhibited by sIPC. The expression level of IKK-β and intranuclear p65 in renal tissues were increased in the IR group while sIPC had exhibited the function of depressing the increased expression levels of IKK-β and intranuclear p65. Compared with the IR group, the expression level of IL-10 of serum and renal tissues in the sIPC group were increased.
sIPC exhibited a potent anti-inflammatory capacity to attenuated renal IR injury.
探讨脾缺血预处理（sIPC）对肾缺血再灌注（IR）损伤的治疗作用。
18 只成年雄性 Sprague Dawley（SD）大鼠行 45 分钟肾缺血，随后再灌注 24 小时。在 sIPC 组，在肾缺血前进行了 3 个周期的脾缺血预处理，包括 5 分钟缺血和 5 分钟再灌注。24 小时后采集血样和肾组织。检测血清 Cr 和 BUN 水平以评估肾功能。通过苏木精-伊红（HE）染色观察缺血再灌注肾脏的形态变化。通过 ELISA 和 Western Blotting 检测血清和肾组织中促炎细胞因子 TNF-α和 IL-6 的水平。此外，还测量了肾组织中 IKK-β、核内 NF-κB、p65 和 IL-10 的水平。
结果表明，IR 组 Cr 和 BUN 水平升高，sIPC 组降低。HE 染色显示 sIPC 减轻了肾缺血再灌注引起的损伤，sIPC 组的肾损伤评分较低。ELISA 和 Western Blotting 结果表明，sIPC 抑制了 IR 引起的 TNF-α和 IL-6 的产生和分泌。IR 组肾组织中 IKK-β 和核内 p65 的表达水平升高，而 sIPC 具有抑制 IKK-β 和核内 p65 表达水平升高的作用。与 IR 组相比，sIPC 组血清和肾组织中 IL-10 的表达水平升高。
sIPC 具有抗炎作用，可减轻肾 IR 损伤。
