铁力木花中二氢黄酮的抗毒力活性
Anti-virulence activities of biflavonoids from Mesua ferrea L. flower.
作者信息
Zhang Xiaochun, Gao Rongrong, Liu Yan, Cong Yuhe, Zhang Dongdong, Zhang Yu, Yang Xuefei, Lu Chunhua, Shen Yuemao
机构信息
Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University.
Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences.
出版信息
Drug Discov Ther. 2019;13(4):222-227. doi: 10.5582/ddt.2019.01053.
Based on the anti-virulence activity on Salmonella, the ethyl acetate extract (EAE) of Mesua ferrea flower was investigated for its chemical constituents. Ten purified compounds were identified and assayed for their inhibitory activity against Type III secretion system (T3SS) by polyacrylamide gel electrophoresis (SDS-PAGE) and Western blots experiments. We found the biflavonoids, rhusflavanone and mesuaferrone B, exhibited inhibitory effects on the secretion of Salmonella pathogenicity island 1 (SPI-1) effector proteins (SipA, B, C and D) without effecting the bacterial growth. In addition, 5, 6, 6'-trihydroxy-[1,1'-biphenyl]-3,3'-dicarboxylic acid (6) is a new natural product from M. ferrea flower.
基于铁力木花乙酸乙酯提取物（EAE）对沙门氏菌的抗毒力活性，对其化学成分进行了研究。通过聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质免疫印迹实验鉴定了10种纯化化合物，并测定了它们对III型分泌系统（T3SS）的抑制活性。我们发现双黄酮类化合物、漆树黄酮和铁力木酮B对沙门氏菌致病岛1（SPI-1）效应蛋白（SipA、B、C和D）的分泌具有抑制作用，而不影响细菌生长。此外，5,6,6'-三羟基-[1,1'-联苯]-3,3'-二羧酸（6）是从铁力木花中分离得到的新天然产物。
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