Department of Rheumatology and Immunology, The First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang 110001, China.
Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang 110001, China.
Mediators Inflamm. 2019 Aug 26;2019:9139145. doi: 10.1155/2019/9139145. eCollection 2019.
IL-35 was reported as a crucial anti-inflammatory cytokine and could efficiently regulate bone metabolism in murine collagen-induced arthritis model. However, the relationship between IL-35 and bone health in human rheumatoid arthritis (RA) has not been clarified. In this study, the aim was to explore the correlations between IL-35 and bone loss in postmenopausal women with RA.
The study included 76 postmenopausal women with RA and 53 healthy postmenopausal women as healthy controls (HCs). Serum IL-35 levels were detected by enzyme-linked immunosorbent assay. Bone mineral density (BMD) at lumbar spine 1-4 and at total hip was measured using dual-energy X-ray absorptiometry. Alkaline phosphatase (ALP), -isomerised carboxy-terminal cross-linking telopeptide of type I collagen (-CTX), and 25-(OH) VitD were measured by turbidimetric inhibition immunoassay.
Serum IL-35 levels were increased compared with HCs, and it positively correlated with BMD and 25-(OH) VitD and negatively correlated with -CTX in postmenopausal women with RA. Furthermore, serum IL-35 levels in the increased ALP group were higher than those in the normal ALP group.
IL-35, an important anti-inflammatory cytokine, may participate in the pathogenesis of bone loss in postmenopausal women with RA.
IL-35 被报道为一种关键的抗炎细胞因子，能够有效调节鼠胶原诱导性关节炎模型中的骨代谢。然而，IL-35 与人类类风湿关节炎（RA）患者的骨骼健康之间的关系尚未阐明。本研究旨在探讨绝经后 RA 患者中 IL-35 与骨丢失之间的相关性。
本研究纳入了 76 例绝经后 RA 患者和 53 例健康绝经后妇女作为健康对照组（HCs）。采用酶联免疫吸附试验检测血清 IL-35 水平。采用双能 X 线吸收法测量腰椎 1-4 及全髋关节的骨密度（BMD）。采用比浊抑制免疫法测量碱性磷酸酶（ALP）、I 型胶原 N 端肽羧基端交联片段（-CTX）和 25-羟维生素 D[25-(OH) VitD]。
与 HCs 相比，绝经后 RA 患者的血清 IL-35 水平升高，且与 BMD 和 25-(OH) VitD 呈正相关，与 -CTX 呈负相关。此外，在 ALP 升高组中，血清 IL-35 水平高于正常 ALP 组。
作为一种重要的抗炎细胞因子，IL-35 可能参与绝经后 RA 患者骨丢失的发病机制。
