Osterman J, Hammond J M
Horm Metab Res. 1979 Aug;11(8):485-8. doi: 10.1055/s-0028-1092767.
Epidermal growth factor, a potent mitrogen for granulosa cells produced a three-fold stimulation of ornithine decarboxylase activity in porcine granulose cells in vitro. Fibroblast growth factor, another compound with mitogenic activity for granulose cells, did not stimulate ornithine decarboxylase. Maximally effective concentrations of a commercial preparation of bovine serum albumin equalled the maximal effect of epidermal growth factor on this enzyme activity. The dominant stimulator(s) in the albumin preparation eluted after bovine serum albumin in gel filtration. At maximally effective concentrations, luteinizing hormone produced substantially greater stimulation than either epidermal growth factor or the bovine albumin preparation. Combinations of saturating doses of any two of these stimulators produced additive effects on enzyme activity.
表皮生长因子是一种对颗粒细胞有强大促有丝分裂作用的物质，它在体外能使猪颗粒细胞中的鸟氨酸脱羧酶活性提高三倍。成纤维细胞生长因子是另一种对颗粒细胞有促有丝分裂活性的化合物，但它不会刺激鸟氨酸脱羧酶。一种市售牛血清白蛋白制剂的最大有效浓度等同于表皮生长因子对该酶活性的最大作用。在凝胶过滤中，白蛋白制剂中的主要刺激物在牛血清白蛋白之后洗脱。在最大有效浓度时，促黄体生成素产生的刺激作用比表皮生长因子或牛白蛋白制剂都要大得多。这些刺激物中任意两种饱和剂量的组合对酶活性产生相加效应。
