Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Tulancingo, Hidalgo 43600, México (ORCID: https://orcid.org/0000-0003-3466-8677 [V.V.-S.]).
Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca 50200, México.
J Food Prot. 2019 Oct;82(10):1706-1713. doi: 10.4315/0362-028X.JFP-18-545.
The members of the genus are important foodborne pathogens, with a worldwide distribution. Wild rainbow trout, from the national protected area Santuario del Agua State Park, Corral de Piedra, were analyzed. Species of were isolated from the trout, and their pathogenic potential was analyzed based on different pathogenicity and virulence factors. The isolates were identified as ( = 15), ( = 8), ( = 3), ( = 2), ( = 2), and ( = 1), by RNA polymerase sigma factor () gene sequencing. Sequence similarity with the type strain was 92.2 to 99.6% for isolates, 97.8 to 98.0% for isolates 99.2% for the isolate, 99.2 to 100% for isolates, and 98.2 to 99.2% for isolates. Notably, isolates A30T2-gills and A30T2-spleen showed sequence similarity of 98.0% with strain CECT 4232T and 99.0% with strain P2G1T. Virulence genes were detected by PCR at the following frequencies: and serine protease, 96.77%; 93.54%; 87.09%; lipases, 74.19%; and 67.74%; 61.29%; 41.93%; 38.70%; 32.26%; 6.46%; 9.67%; and 3.23%. These results indicate that several species had the potential pathogenicity to infect wild rainbow trout in the waterway created by the Corral de Piedra dam, suggesting they could be an emerging zoonotic pathogen.
该属的成员是重要的食源性病原体，分布于世界各地。对来自国家保护区 Santuario del Agua 州立公园、Corral de Piedra 的野生虹鳟鱼进行了分析。从这些鳟鱼中分离出了 属的物种，并根据不同的致病性和毒力因子分析了它们的致病潜力。通过 RNA 聚合酶 sigma 因子（）基因测序，将这些分离株鉴定为 （=15）、（=8）、（=3）、（=2）、（=2）和 （=1）。与模式株的序列相似性为 92.2%至 99.6%的 分离株、97.8%至 98.0%的 分离株、99.2%的 分离株、99.2%至 100%的 分离株和 98.2%至 99.2%的 分离株。值得注意的是，A30T2-鳃和 A30T2-脾分离株与菌株 CECT 4232T 的序列相似性为 98.0%，与菌株 P2G1T 的序列相似性为 99.0%。通过 PCR 检测到毒力基因的频率如下：和丝氨酸蛋白酶，96.77%； 93.54%； 87.09%；脂肪酶，74.19%； 和 67.74%； 61.29%； 41.93%； 38.70%； 32.26%； 6.46%； 9.67%；和 3.23%。这些结果表明，一些 种具有感染 Corral de Piedra 水坝形成的水域中野生虹鳟鱼的潜在致病性，表明它们可能是一种新兴的人畜共患病病原体。
