Hirohashi S
Pathology Division, National Cancer Center Research Institute.
Hum Cell. 1988 Mar;1(1):17-9.
Remarkable progresses have been made in the field of oncogenes in the last several years. More than 40 oncogenes or proto-oncogenes were identified by transfection assay and by weak homology of base sequences with known oncogenes. Many of them were shown to play a specific role in regulation of cell growth and signal transduction, but their exact roles in development and progression of human cancers are still not clear. Study of oncogenes and their expression at cellular level using immunohistochemistry and in situ hybridization will contribute to understand how oncogenes are involved in the multiple steps of carcinogenesis. In this article, application of newly established monoclonal antibodies to ras p21 for immunohistochemistry and immunoblotting analysis and possibilities of DNA analysis using formalin-fixed paraffin-embedded blocks are discussed.
在过去几年中，癌基因领域取得了显著进展。通过转染试验以及与已知癌基因的碱基序列微弱同源性，已鉴定出40多种癌基因或原癌基因。其中许多已被证明在细胞生长调节和信号转导中发挥特定作用，但其在人类癌症发生和发展中的确切作用仍不清楚。利用免疫组织化学和原位杂交在细胞水平上研究癌基因及其表达，将有助于理解癌基因如何参与致癌作用的多个步骤。本文讨论了新建立的针对ras p21的单克隆抗体在免疫组织化学和免疫印迹分析中的应用，以及使用福尔马林固定石蜡包埋块进行DNA分析的可能性。
