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理解比目鱼（塞内加尔鳎）中的拟白化现象：一种转录组学和宏基因组学方法。

Understanding pseudo-albinism in sole (Solea senegalensis): a transcriptomics and metagenomics approach.

机构信息

Centre of Marine Sciences (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal.

LifeSequencing-ADM Nutrition, Parc Cientific Universidad De Valencia, Edif. 2, C/Catedrático Agustín Escardino Benlloch, 9, 46980, Paterna, Spain.

出版信息

Sci Rep. 2019 Sep 20;9(1):13604. doi: 10.1038/s41598-019-49501-6.

DOI:10.1038/s41598-019-49501-6
PMID:31541115
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6754371/
Abstract

Pseudo-albinism is a pigmentation disorder observed in flatfish aquaculture with a complex, multi-factor aetiology. We tested the hypothesis that pigmentation abnormalities are an overt signal of more generalised modifications in tissue structure and function, using as a model the Senegalese sole and two important innate immune barriers, the skin and intestine, and their microbiomes. Stereological analyses in pseudo-albino sole revealed a significantly increased mucous cell number in skin (P < 0.001) and a significantly thicker muscle layer and lamina propria in gut (P < 0.001). RNA-seq transcriptome analysis of the skin and gut identified 573 differentially expressed transcripts (DETs, FDR < 0.05) between pseudo-albino and pigmented soles (one pool/tissue from 4 individuals/phenotype). DETs were mainly linked to pigment production, skin structure and regeneration and smooth muscle contraction. The microbiome (16 S rRNA analysis) was highly diverse in pigmented and pseudo-albino skin but in gut had low complexity and diverged between the two pigmentation phenotypes. Quantitative PCR revealed significantly lower loads of Mycoplasma (P < 0.05) and Vibrio bacteria (P < 0.01) in pseudo-albino compared to the control. The study revealed that pseudo-albinism in addition to pigmentation changes was associated with generalised changes in the skin and gut structure and a modification in the gut microbiome.

摘要

假性白化病是一种在比目鱼养殖中观察到的色素沉着障碍，具有复杂的多因素病因。我们通过塞内加尔比目鱼和两个重要的先天免疫屏障——皮肤和肠道及其微生物组来检验假说，即色素沉着异常是组织结构和功能更广泛改变的明显信号。在假性白化比目鱼中进行的体视学分析显示，皮肤中的粘液细胞数量显著增加（P<0.001），肠道中的肌肉层和固有层明显增厚（P<0.001）。皮肤和肠道的 RNA-seq 转录组分析鉴定出 573 个差异表达转录本（DETs，FDR<0.05），在假性白化和有色比目鱼之间（每个表型 4 个个体/池/组织）。DETs 主要与色素生成、皮肤结构和再生以及平滑肌收缩有关。微生物组（16S rRNA 分析）在有色和假性白化皮肤中高度多样化，但在肠道中复杂性较低，在两种色素沉着表型之间存在差异。定量 PCR 显示，与对照组相比，假性白化中的支原体（P<0.05）和弧菌（P<0.01）载量显著降低。该研究表明，除了色素沉着变化外，假性白化还与皮肤和肠道结构的普遍变化以及肠道微生物组的改变有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/7c77f3dfc137/41598_2019_49501_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/f702efba5b6c/41598_2019_49501_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/59b0f856028e/41598_2019_49501_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/b62649d096bf/41598_2019_49501_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/c38c7910053e/41598_2019_49501_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/0f4b4e7789fb/41598_2019_49501_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/93abbf7d91c7/41598_2019_49501_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/7c77f3dfc137/41598_2019_49501_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/f702efba5b6c/41598_2019_49501_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/59b0f856028e/41598_2019_49501_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/b62649d096bf/41598_2019_49501_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/c38c7910053e/41598_2019_49501_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/0f4b4e7789fb/41598_2019_49501_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/93abbf7d91c7/41598_2019_49501_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3c1/6754371/7c77f3dfc137/41598_2019_49501_Fig7_HTML.jpg

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