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细菌产物紫霉素通过 TLR8 发挥免疫刺激作用。

The Bacterial Product Violacein Exerts an Immunostimulatory Effect Via TLR8.

机构信息

Escuela de Química, Universidad de Costa Rica, 11501-2060, San José, Costa Rica.

Centro de Investigaciones en Productos Naturales (CIPRONA), Universidad de Costa Rica, 11501-2060, San José, Costa Rica.

出版信息

Sci Rep. 2019 Sep 20;9(1):13661. doi: 10.1038/s41598-019-50038-x.

DOI:10.1038/s41598-019-50038-x
PMID:31541142
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6754391/
Abstract

Violacein, an indole-derived, purple-colored natural pigment isolated from Chromobacterium violaceum has shown multiple biological activities. In this work, we studied the effect of violacein in different immune cell lines, namely THP-1, MonoMac 6, ANA-1, Raw 264.7 cells, as well as in human peripheral blood mononuclear cells (PBMCs). A stimulation of TNF-α production was observed in murine macrophages (ANA-1 and Raw 264.7), and in PBMCs, IL-6 and IL-1β secretion was detected. We obtained evidence of the molecular mechanism of activation by determining the mRNA expression pattern upon treatment with violacein in Raw 264.7 cells. Incubation with violacein caused activation of pathways related with an immune and inflammatory response. Our data utilizing TLR-transfected HEK-293 cells indicate that violacein activates the human TLR8 (hTLR8) receptor signaling pathway and not human TLR7 (hTLR7). Furthermore, we found that the immunostimulatory effect of violacein in PBMCs could be suppressed by the specific hTLR8 antagonist, CU-CPT9a. Finally, we studied the interaction of hTLR8 with violacein in silico and obtained evidence that violacein could bind to hTLR8 in a similar fashion to imidazoquinoline compounds. Therefore, our results indicate that violacein may have some potential in contributing to future immune therapy strategies.

摘要

从 Chromobacterium violaceum 中分离得到的吲哚衍生的紫色天然色素——violacein 具有多种生物学活性。在这项工作中，我们研究了 violacein 在不同免疫细胞系中的作用，包括 THP-1、MonoMac 6、ANA-1、Raw 264.7 细胞以及人外周血单核细胞（PBMCs）中的作用。我们观察到 violacein 刺激了鼠源巨噬细胞（ANA-1 和 Raw 264.7）中 TNF-α的产生，以及 PBMCs 中 IL-6 和 IL-1β的分泌。通过在 Raw 264.7 细胞中处理 violacein 来确定其mRNA 表达模式，我们获得了激活的分子机制的证据。violacein 的孵育导致与免疫和炎症反应相关的途径被激活。我们利用 TLR 转染的 HEK-293 细胞的数据表明，violacein 激活了人 TLR8（hTLR8）受体信号通路，而不是人 TLR7（hTLR7）。此外，我们发现 violacein 在 PBMCs 中的免疫刺激作用可以被特异性 hTLR8 拮抗剂 CU-CPT9a 抑制。最后，我们在计算机上研究了 hTLR8 与 violacein 的相互作用，并获得了 violacein 可以以类似于咪唑并喹啉化合物的方式与 hTLR8 结合的证据。因此，我们的结果表明 violacein 可能在未来的免疫治疗策略中具有一定的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/4bc97943a068/41598_2019_50038_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/600e1d2be84b/41598_2019_50038_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/c57307dcbb02/41598_2019_50038_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/195a6c5e59fa/41598_2019_50038_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/e57ec01a7ad1/41598_2019_50038_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/9fcbf48d1e2d/41598_2019_50038_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/8170b05108fc/41598_2019_50038_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/4bc97943a068/41598_2019_50038_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/600e1d2be84b/41598_2019_50038_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/c57307dcbb02/41598_2019_50038_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/195a6c5e59fa/41598_2019_50038_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/e57ec01a7ad1/41598_2019_50038_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/9fcbf48d1e2d/41598_2019_50038_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/8170b05108fc/41598_2019_50038_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0636/6754391/4bc97943a068/41598_2019_50038_Fig7_HTML.jpg

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2
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Nat Chem Biol. 2018 Jan;14(1):58-64. doi: 10.1038/nchembio.2518. Epub 2017 Nov 20.
3
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J Cancer Res Clin Oncol. 2023 Oct;149(13):10975-10987. doi: 10.1007/s00432-023-04930-9. Epub 2023 Jun 4.
4
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Front Oncol. 2022 May 11;12:872223. doi: 10.3389/fonc.2022.872223. eCollection 2022.
5
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RSC Adv. 2020 Aug 10;10(49):29336-29346. doi: 10.1039/d0ra05101b. eCollection 2020 Aug 5.
6
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