紫菌素对表皮葡萄球菌的抗菌活性及对市售抗生素的协同作用。
Violacein antimicrobial activity on Staphylococcus epidermidis and synergistic effect on commercially available antibiotics.
作者信息
Dodou H V, de Morais Batista A H, Sales G W P, de Medeiros S C, Rodrigues M L, Nogueira P C N, Silveira E R, Nogueira N A P
机构信息
Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Brazil.
Department of Organic Chemistry, Federal University of Ceará, Fortaleza, Brazil.
出版信息
J Appl Microbiol. 2017 Oct;123(4):853-860. doi: 10.1111/jam.13547.
AIMS
The study aimed to assess whether violacein has antimicrobial activity on Staphylococcus epidermidis and synergistically modulates the action of commercially available antimicrobial drugs.
METHODS AND RESULTS
Violacein showed excellent antimicrobial activity on biofilm-forming and nonbiofilm-forming S. epidermidis strains (ATCC 35984) (ATCC 12228), with bacteriostatic (MIC = 20 μg ml and 10 μg ml respectively) and bactericidal effects (MBC = 20 μg ml for both strains), observed in short periods of exposure. The violacein bactericidal concentration led to S. epidermidis death after 2-3 h of exposure. Additionally, violacein synergistically modulated the activity of different antimicrobial classes on S. epidermidis ATCC 12228 (81·8%; n = 9) and on S. epidermidis ATCC 35984 (54·5%; n = 6), reducing the MIC of these antibiotics by up to 16-fold.
CONCLUSION
Violacein shows excellent antimicrobial activity on S. epidermidis strains.
SIGNIFICANCE AND IMPACT OF THE STUDY
Violacein shows the potential for the development of a new drug for the treatment of infections caused by S. epidermidis.
目的
本研究旨在评估紫菌素对表皮葡萄球菌是否具有抗菌活性，以及是否能协同调节市售抗菌药物的作用。
方法与结果
紫菌素对形成生物膜和未形成生物膜的表皮葡萄球菌菌株（ATCC 35984）（ATCC 12228）显示出优异的抗菌活性，在短时间暴露下观察到抑菌作用（MIC分别为20μg/ml和10μg/ml）和杀菌作用（两种菌株的MBC均为20μg/ml）。紫菌素的杀菌浓度在暴露2至3小时后导致表皮葡萄球菌死亡。此外，紫菌素协同调节了不同抗菌类别对表皮葡萄球菌ATCC 12228（81.8%；n = 9）和表皮葡萄球菌ATCC 35984（54.5%；n = 6）的活性，将这些抗生素的MIC降低了多达16倍。
结论
紫菌素对表皮葡萄球菌菌株显示出优异的抗菌活性。
研究的意义和影响
紫菌素显示出开发用于治疗由表皮葡萄球菌引起的感染的新药的潜力。
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