Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
Academic Department of Neurosciences, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, UK.
J Cell Physiol. 2020 Apr;235(4):3474-3484. doi: 10.1002/jcp.29236. Epub 2019 Sep 20.
In different retrospective studies, a protective role of regional anesthetics in reducing cancer recurrence after surgery was indicated. Accordingly, it has been previously demonstrated a protective effect of anesthetics in breast cancer cells and in other types of cancer. On the other hand, how anesthetics influence cancer needs in-depth investigations. For this purpose, two different human cancer cell lines, MDA-MB-231, triple-negative breast cancer, and A375, melanoma, were used in this study. By means of Western blotting and immunofluorescence and terminal deoxynucleotidyl transferase dUTP nick end labeling analyses, the signal transduction pathways activated by the anesthetics, such as ropivacaine and levobupivacaine, were analyzed. The data obtained demonstrated that both anesthetics are able to counteract cell proliferation by positively modulating cell death signaling and by decreasing cell proliferation and survival pathways.
在不同的回顾性研究中，局部麻醉剂在减少手术后癌症复发方面显示出保护作用。因此，先前已经证明了麻醉剂对乳腺癌细胞和其他类型的癌症具有保护作用。另一方面，麻醉剂如何影响癌症需要深入研究。为此，本研究使用了两种不同的人癌细胞系，MDA-MB-231（三阴性乳腺癌）和 A375（黑色素瘤）。通过 Western blot 分析、免疫荧光分析和末端脱氧核苷酸转移酶 dUTP 缺口末端标记分析，分析了麻醉剂（如罗哌卡因和左布比卡因）激活的信号转导通路。获得的数据表明，两种麻醉剂都能够通过正向调节细胞死亡信号来抑制细胞增殖，并通过降低细胞增殖和存活途径来抑制细胞增殖。
