Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University (TMU), PO Box: 14115-143, Iran.
Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University (TMU), PO Box: 14115-143, Iran.
J Environ Manage. 2019 Dec 1;251:109554. doi: 10.1016/j.jenvman.2019.109554. Epub 2019 Sep 18.
In this study, performance of biotrickling filters (BTFs) inoculated with fungus Phanerochaete chrysosporium at 30 °C and 40 °C in the absence and presence of silicone oil (10% v/v) was investigated. Removal of toluene was carried out at empty bed residence time (EBRT) of 1 min and at inlet concentrations of 0.5-4.4 g m and 0.5-24.7 g m for one-liquid phase (OLP-BTF) and two-liquid phase BTF (TLP-BTF), respectively. In general, at 40 °C, removal efficiencies (REs) > 80% were obtained in OLP-BTF for the inlet toluene concentrations < 2.5 g m, and REs > 70% were obtained for concentrations < 18 g m in TLP-BTF. Based on the balanced equation for biodegradation, fungal respiration produced more CO in OLP-BTF (1.38 mol CO/mole toluene) in comparison to TLP-BTF (0.67 mol CO/mole toluene). In other words, the presence of oil enhanced microbial growth due to the increase of hydrophobic substrate bioavailability. The activity of extracellular ligninolytic manganese peroxidase (MnP) enzyme produced by the fungal culture was detected in the range of 27.6-71.6 U L (μmol min L) at 40 °C in TLP-BTF, while no enzymatic activity was detected in OLP-BTF.
本研究考察了在 30°C 和 40°C 下，真菌糙皮侧耳（Phanerochaete chrysosporium）接种的生物滴滤塔（BTF）在无和存在硅油（10%v/v）时的性能。在空床停留时间（EBRT）为 1 分钟，入口浓度分别为 0.5-4.4 g/m 和 0.5-24.7 g/m 时，采用一相（OLP-BTF）和两相（TLP-BTF）生物滴滤塔去除甲苯。一般来说，在 40°C 下，对于入口甲苯浓度<2.5 g/m 的 OLP-BTF，去除效率（RE）>80%，对于入口甲苯浓度<18 g/m 的 TLP-BTF，RE>70%。根据生物降解的平衡方程，真菌呼吸在 OLP-BTF 中产生了更多的 CO（1.38 mol CO/摩尔甲苯），而在 TLP-BTF 中产生了 0.67 mol CO/摩尔甲苯。换句话说，由于疏水性底物生物利用度的增加，油的存在促进了微生物的生长。在 TLP-BTF 中，40°C 时真菌培养物产生的胞外木质素过氧化物酶（MnP）酶的活性范围为 27.6-71.6 U L（μmol min L），而在 OLP-BTF 中则没有检测到酶活性。
