Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA, 16802, USA.
Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA, 16802, USA.
Curr Opin Insect Sci. 2019 Dec;36:90-95. doi: 10.1016/j.cois.2019.08.013. Epub 2019 Aug 30.
Expanding genome sequencing and transgenic technologies are enabling the discovery of genes driving phenotypic diversity across insect taxa. Limitations in downstream functional genetic approaches, however, have been an obstacle for developing non-model systems for evolutionary genetics. Phenotypically diverse radiations, such as those exhibiting convergence and divergence as a result of mimicry, are ideal for evolutionary genetics as they can lead to insights using comparative genomic approaches alone. The varied and repeated instances of phenotypes in highly polymorphic systems allow assessment of whether similar loci are repeatedly targeted by selection and can inform how alleles sort across lineages. Comparative genomics of these taxa can be used to decipher components of gene regulatory networks, dissect regulatory regions, and validate genes.
扩展基因组测序和转基因技术使人们能够发现驱动昆虫分类群表型多样性的基因。然而，下游功能遗传方法的局限性一直是为进化遗传学开发非模式系统的障碍。表型多样化的辐射，如由于模拟而表现出趋同和分歧的辐射，非常适合进化遗传学，因为它们可以仅使用比较基因组方法来提供见解。高度多态系统中多样化和重复的表型实例可以评估是否有类似的基因座被选择反复靶向，并可以说明等位基因如何在谱系中排列。对这些分类群进行比较基因组学可以用于破译基因调控网络的组成部分、剖析调控区域和验证基因。
