Li Chang, Zhang Xu, Lu Yin, Fan Zheng, Wang Tiecheng, Zhang Guoliang
Institute of Oceanic and Environmental Chemical Engineering, State Key Lab Breeding Base of Green Chemical Synthesis Technology, Zhejiang University of Technology, Hangzhou, 310014, China.
College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, China.
J Hazard Mater. 2020 Feb 5;383:121198. doi: 10.1016/j.jhazmat.2019.121198. Epub 2019 Sep 10.
In this study, we discovered and isolated a new genus Brevibacillus strain from effluent of dyeing and finishing factory containing highly toxic p-chloroanilines (PCA). Based on the morphological, physiological and biochemical characteristics, as well as 16S rDNA sequence, the strain was identified and denominated as Brevibacillus S-618. Co-metabolism effect was found with extra carbon sources including sodium succinate, sodium citrate, ammonium chloride and glucose which can efficiently promote the biodegradation process of PCA. Under the optimal growth conditions at temperature of 30 °C, pH˜7 and air-water ratio of 0.3 m/m·min, the degradation rate of PCA in a 2 L pilot bioreactor with high concentration of 180 mg/L increased from 86.7% to 100% within 72 h after adding sodium succinate. The release of chloride ions during the growth process of the strain was equivalent to the degradation amount of PCA. Meanwhile, the cleavage pathway of PCA degradation by Brevibacillus S-618 was proposed by analysis of enzyme activities of microorganism and intermediate products in the reaction. Benefiting from excellent degradation ability and unique characters in high pollutant contents, high efficient bioreactor can easily be scale up for industrial application. Our study provides a facile route for cost-effectively and environmental-friendly degrading hazardous chemicals.
在本研究中，我们从含有高毒性对氯苯胺（PCA）的印染厂废水中发现并分离出一株新的短芽孢杆菌属菌株。基于形态学、生理学和生化特性以及16S rDNA序列，该菌株被鉴定并命名为短芽孢杆菌S-618。研究发现，包括琥珀酸钠、柠檬酸钠、氯化铵和葡萄糖在内的额外碳源具有共代谢作用，能够有效促进PCA的生物降解过程。在30℃、pH值约为7、气水比为0.3 m/m·min的最佳生长条件下，在添加琥珀酸钠后72小时内，2 L中试生物反应器中高浓度180 mg/L的PCA降解率从86.7%提高到了100%。该菌株生长过程中氯离子的释放量与PCA的降解量相当。同时，通过分析微生物的酶活性和反应中的中间产物，提出了短芽孢杆菌S-618降解PCA的裂解途径。得益于其出色的降解能力和在高污染物含量环境中的独特特性，高效生物反应器可轻松扩大规模用于工业应用。我们的研究为经济高效且环保地降解有害化学品提供了一条简便途径。
