Huang Shicong, Gao Jiahui, Zhou Lin, Gao Liujian, Song Mengke, Zeng Qiaoyun
College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Toxics. 2025 Jun 16;13(6):506. doi: 10.3390/toxics13060506.
Efficient methods to remediate PCA (p-chloroaniline)-polluted environments are urgent due to the widespread persistence and toxicity of PCA in the environment. Microbial degradation presents a promising approach for remediating PCA pollution. However, the PCA-degrading fungi still have yet to be explored. This study confirmed the highly PCA-degrading efficiency of an isolated fungus, SP535. This fungus can achieve a PCA degradation efficiency of 100% under optimal conditions characterized by an initial PCA concentration of 1.0 mM, pH of 7.0 and a temperature of 25 °C. SEM and TEM analyses revealed that the toxicity of PCA resulted in roughened surfaces of SP535 hyphae, voids in the cytoplasm, and thickened cell walls. PCA addition significantly elevated the activities of cytochrome P450 monooxygenase in both cell-free extracts and microsomal fractions in the media, suggesting the important role of the P450 system in PCA metabolization by SP535. The results provide a microbial resource and fundamental knowledge for addressing PCA pollution.
由于对氯苯胺（PCA）在环境中广泛存在且具有持久性和毒性，因此迫切需要有效的方法来修复受PCA污染的环境。微生物降解是一种很有前景的PCA污染修复方法。然而，降解PCA的真菌仍有待探索。本研究证实了分离出的真菌SP535具有很高的PCA降解效率。在初始PCA浓度为1.0 mM、pH为7.0、温度为25°C的最佳条件下，这种真菌能够实现100%的PCA降解效率。扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析表明，PCA的毒性导致SP535菌丝表面粗糙、细胞质出现空洞以及细胞壁增厚。添加PCA显著提高了培养基中无细胞提取物和微粒体组分中细胞色素P450单加氧酶的活性，这表明P450系统在SP535代谢PCA过程中发挥着重要作用。这些结果为解决PCA污染提供了一种微生物资源和基础知识。
