Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, 200241 Shanghai, China; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1, Xujiaping, 730046 Lanzhou, Gansu Province, China.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, 200241 Shanghai, China.
Acta Trop. 2020 Jan;201:105184. doi: 10.1016/j.actatropica.2019.105184. Epub 2019 Sep 19.
Schistosomiasis is a chronic parasitic disease caused by the genus Schistosoma and poses a great threat to human and animal health. Identification of effective biomarkers would facilitate evaluation of drug efficacy and recognition of infected hosts, which are crucial for effective schistosomiasis control. Extracellular vesicle (EV) proteins are considered ideal biomarkers for developing invasive diagnostic tools. In this study, we evaluated the potential of Schistosoma japonicum EV (SjEV) proteins as biomarkers for diagnosing schistosomiasis. Several SjEV proteins were subject to epitope prediction using DNASTAR software, and the diagnostic potential of selected peptides was evaluated using enzyme-linked immunosorbent assay (ELISA). The results indicated that the sera showed detectable antibody levels against the two antigens in mice, rabbits, and humans infected with S. japonicum. Further analysis of the combined epitope protein demonstrated a modest sensitivity for detection of Schistosomiasis japonica. Our preliminary study suggests that S. japonicum EV proteins could serve as potential biomarkers for developing diagnostic tools for schistosomiasis.
血吸虫病是一种由血吸虫属引起的慢性寄生虫病，对人类和动物的健康构成严重威胁。鉴定有效的生物标志物将有助于评估药物疗效和识别感染宿主，这对于有效控制血吸虫病至关重要。细胞外囊泡 (EV) 蛋白被认为是开发侵袭性诊断工具的理想生物标志物。在这项研究中，我们评估了日本血吸虫 EV（SjEV）蛋白作为诊断血吸虫病的生物标志物的潜力。使用 DNASTAR 软件对几种 SjEV 蛋白进行了表位预测，并用酶联免疫吸附试验 (ELISA) 评估了选定肽的诊断潜力。结果表明，感染日本血吸虫的小鼠、兔和人中的血清对两种抗原均显示出可检测的抗体水平。对组合表位蛋白的进一步分析表明，该方法对检测日本血吸虫病具有一定的敏感性。我们的初步研究表明，日本血吸虫 EV 蛋白可用作开发血吸虫病诊断工具的潜在生物标志物。
