Shanghai Tenth People's Hospital, Institute for Infectious Diseases and Vaccine Development, Tongji University School of Medicine, Shanghai, 200331, China; School of Biotechnology Jiangsu University of Science and Technology, Zhen Jiang, 212100, China.
Department of Zoology, Utkal University, Bhubaneswar, Odisha, 751004, India.
Exp Parasitol. 2024 Jul;262:108776. doi: 10.1016/j.exppara.2024.108776. Epub 2024 May 14.
Timely and accurate diagnosis of Schistosoma infection is important to adopt effective strategies for schistosomiasis control. Previously, we demonstrated that Schistosoma japonicum can secret extracellular vesicles and their cargos may serve as a novel type of biomarkers for diagnosing schistosomiasis. Here, we developed a Gaussia luciferase immunoprecipitation assay combined with S. japonicum extracellular vesicle (SjEV) protein to evaluate its potential for diagnosing schistosomiasis. A saposin-like protein (SjSLP) identified from SjEVs was fused to the Gaussia luciferase as the diagnostic antigen. The developed method showed good capability for detecting S. japonicum infection in mice and human patients. We also observed that the method could detect Schistosoma infection in mice as early as 7 days of post-infection, which showed better sensitivity than that of indirect ELISA method. Overall, the developed method showed a good potential for detecting Schistosoma infection particularly for early stage, which may provide an alternative strategy for identify Schistosoma infection for disease control.
及时准确地诊断血吸虫感染对于采取有效的血吸虫病控制策略非常重要。此前，我们证明日本血吸虫可以分泌细胞外囊泡，其携带的货物可能作为诊断血吸虫病的新型生物标志物。在这里，我们开发了一种基于 Gaussia 荧光素酶免疫沉淀检测联合日本血吸虫细胞外囊泡（SjEV）蛋白的方法，以评估其在诊断血吸虫病方面的潜力。从 SjEVs 中鉴定出一种类脂转移蛋白（SjSLP），并将其与 Gaussia 荧光素酶融合作为诊断抗原。所开发的方法显示出在小鼠和人类患者中检测日本血吸虫感染的良好能力。我们还观察到，该方法可以在感染后 7 天内检测到小鼠的血吸虫感染，其灵敏度高于间接 ELISA 方法。总的来说，所开发的方法显示出了检测血吸虫感染的良好潜力，特别是在早期阶段，这可能为疾病控制提供了一种替代的识别血吸虫感染的策略。
