School of Materials Science and Engineering, Central South University, Changsha, Hunan, 410083, China.
School of Materials Science and Engineering, Central South University, Changsha, Hunan, 410083, China.
Biosens Bioelectron. 2019 Dec 1;145:111713. doi: 10.1016/j.bios.2019.111713. Epub 2019 Sep 17.
We report a paper-based surface-enhanced Raman spectroscopy (SERS) biosensor integrating the enrichment capability, namely enPSERS biosensor, for the sensitive, label-free detection of free bilirubin in blood serum for the accurate diagnosis of jaundice and its related diseases. This biosensor comprises multifunctional graphene oxide-plasmonic gold nanostar (GO-GNS) hybrids decorated on the filter paper, which integrates the high sensitivity of SERS detection, enrichment for serum bilirubin and fluorescence superquenching capability of GO-GNS hybrids for sensitive detection of serum bilirubin. The study of adsorption kinetics reveals that both electrostatic and π-π interactions between the GO-GNS hybrids and targets are responsible for the enrichment of bilirubin, and the adsorption process follows the pseudo-second-order kinetic model. The results of SERS detection of bilirubin in blood serum show two differential linear response ranges from 5.0 to 150 μM and 150-500 μM with the detection limit as low as 0.436 μM. The comparison of the results obtained from our present enPSERS biosensor with the commercial diazo reaction method for determination of free bilirubin in blood serum reveals the clinical effectiveness and suitability of the developed paper-based SERS biosensor. We believe that this sensitive and label-free SERS biosensor holds considerable promise for clinical translation in accurate diagnosis of jaundice.
我们报告了一种基于纸的表面增强拉曼光谱（SERS）生物传感器，该传感器集成了富集能力，即 enPSERS 生物传感器，用于灵敏、无标记检测血清中的游离胆红素，以准确诊断黄疸及其相关疾病。该生物传感器由多功能氧化石墨烯-等离子体金纳米星（GO-GNS）杂化物修饰在滤纸上组成，它集成了 SERS 检测的高灵敏度、血清胆红素的富集以及 GO-GNS 杂化物的荧光超猝灭能力，用于灵敏检测血清胆红素。吸附动力学研究表明，GO-GNS 杂化物与靶标之间的静电和π-π相互作用都有助于胆红素的富集，吸附过程遵循准二级动力学模型。血清中胆红素的 SERS 检测结果表明，两个差分线性响应范围分别为 5.0 至 150μM 和 150 至 500μM，检测限低至 0.436μM。与商业重氮反应法测定血清中游离胆红素的结果相比，我们目前的 enPSERS 生物传感器的结果表明，开发的基于纸的 SERS 生物传感器具有临床有效性和适用性。我们相信，这种灵敏、无标记的 SERS 生物传感器在黄疸的准确诊断中具有很大的临床转化潜力。
