Hematology/Oncology, Children's Hospital of Michigan, Wayne State University, Detroit, Michigan, USA
Hematology/Oncology, Children's Hospital of Michigan, Wayne State University, Detroit, Michigan, USA.
J Clin Pathol. 2020 Mar;73(3):176-179. doi: 10.1136/jclinpath-2019-205967. Epub 2019 Sep 21.
To compare immunological microenvironments in local and distant lymphoid tissues in Hodgkin's lymphoma (HL) in children.
We have analysed diagnostic bone marrow (BM) samples in 22 and corresponding involved lymph node (LN) in eight and peripheral blood (PB) in eight cases of HL by flow cytometry and sought correlations with clinical features retrospectively.
While there were significant differences in lymphocyte compositions of BM and LN tissues, the distribution of lymphocyte subsets mimicked each other in BM and PB. CD8-positive cytotoxic T cells predominate the bone marrow in contrast to CD4-positive helper T cells in LN tissue with corresponding CD4/CD8 ratios (0.85 and 5.3, respectively; p=0.002). Additionally, T-large granular lymphocytes population was much higher in BM in comparison to LN tissue (10.5% vs 4.5%; p=0.036).
Local immunological microenvironment appears to be highly influenced by HL tumour cells and distant site lymphocyte composition reflects immune response to control the neoplastic process.
比较儿童霍奇金淋巴瘤（HL）局部和远处淋巴组织中的免疫微环境。
我们通过流式细胞术分析了 22 例 HL 患儿的诊断性骨髓（BM）样本、8 例相应受累淋巴结（LN）和 8 例 PB，并回顾性分析了与临床特征的相关性。
虽然 BM 和 LN 组织的淋巴细胞组成存在显著差异，但 BM 和 PB 中淋巴细胞亚群的分布相似。与 LN 组织中的 CD4 阳性辅助性 T 细胞相比，CD8 阳性细胞毒性 T 细胞在 BM 中占优势，相应的 CD4/CD8 比值分别为（0.85 和 5.3；p=0.002）。此外，与 LN 组织相比，BM 中的 T 大颗粒淋巴细胞群体明显更高（10.5% vs 4.5%；p=0.036）。
局部免疫微环境似乎受到 HL 肿瘤细胞的强烈影响，而远处部位的淋巴细胞组成则反映了对控制肿瘤过程的免疫反应。
