Pituch-Noworolska Anna, Drabik Grazyna, Kacińska Ewa, Klekawka Tomasz
Department of Clinical Immunology, Polish-American Children Hospital, Medical College, Jagiellonian University, Cracow, Poland.
Acta Haematol. 2004;112(3):129-35. doi: 10.1159/000079723.
Proliferating Reed-Sternberg and Hodgkin's cells within the lymph node in Hodgkin's disease (HD) affect the surrounding cells, which results in alterations of the proportion between T and B lymphocytes and the activation of these cells. The purpose of this study was to assay the changes within lymphocyte populations in lymph nodes in different types of HD. The lymph nodes of 10 children with nonmalignant, reactive changes, and 31 children with HD of the following types: mixed cellularity (MC; 11 cases), nodular sclerosis (NS; 17 cases) and lymphocyte depletion (LD; 3 cases) were included in the study. The percentage of T lymphocytes (CD3+), B lymphocytes (CD22+), T cell (CD4+ and CD8+) and B cell subsets (kappa+ and lambda+ light chain) were assayed with flow cytometry. The ratio of T:B lymphocytes in reactive lymph nodes and in NS type of HD was similar. An increase of the T cell percentage (resembling an increase of the CD4+ subset) and a decrease of B cells resulted in a high ratio of T:B and was noted in the MC and LD types of HD. These HD types were associated with marked changes in lymphocytes within the lymph node, which suggested a different effect of proliferating Reed-Sternberg and Hodgkin cells in HD on the surrounding cells.
霍奇金淋巴瘤（HD）淋巴结内增殖的里德-施特恩伯格细胞和霍奇金细胞会影响周围细胞，导致T淋巴细胞和B淋巴细胞比例改变以及这些细胞的激活。本研究的目的是分析不同类型HD患者淋巴结中淋巴细胞群体的变化。研究纳入了10例有非恶性反应性改变的儿童的淋巴结，以及31例以下类型HD患儿的淋巴结：混合细胞型（MC；11例）、结节硬化型（NS；17例）和淋巴细胞消减型（LD；3例）。采用流式细胞术检测T淋巴细胞（CD3+）、B淋巴细胞（CD22+）、T细胞亚群（CD4+和CD8+）以及B细胞亚群（κ+和λ+轻链）的百分比。反应性淋巴结和NS型HD中T:B淋巴细胞的比例相似。T细胞百分比增加（类似于CD4+亚群增加）且B细胞减少，导致T:B比例升高，这在MC型和LD型HD中可见。这些HD类型与淋巴结内淋巴细胞的显著变化有关，这表明HD中增殖的里德-施特恩伯格细胞和霍奇金细胞对周围细胞有不同的影响。
