Sen Siladitya, Anand Kukkamalla Meena
Department of Periodontology, MMDCH, Darbhangha, Bihar, India.
Department of Periodontology, Manipal College of Dental Sciences, Manipal, Karnataka, India.
J Indian Soc Periodontol. 2019 Sep-Oct;23(5):409-415. doi: 10.4103/jisp.jisp_406_18.
Diabetes has become an endemic throughout the world. Periodontitis is associated with diabetes, and it has been regarded as the sixth complication of diabetes. However, the role of diabetes on periodontopathic microbiota such as remains unclear.
To compare the total number of different genotypes of in chronic periodontitis patients with and without diabetes by using arbitrarily primed-polymerase chain reaction (AP-PCR) and heteroduplex-PCR.
This is a single-center comparative study.
A total of 100 patients were included in this study. Patients were divided into two groups: Group A - patients having chronic periodontitis without diabetes (50) and Group B - patients having chronic periodontitis with diabetes (50). Subgingival plaque samples were collected from both groups of patients. Plaque samples were cultured for . Positive culture samples and extracted DNA from samples by proteinase-K method were collected. Part of DNA samples were checked by AP-PCR using OPA-13 primer, and part of the DNA were checked using heteroduplex-PCR using -specific Pg8 primer. Results were analyzed by polyacrylamide gel electrophoresis procedure.
Independent -test and Chi-square test (SPSS 16) were used.
Thirty-four out of fifty samples of Group B patients were found positive for , whereas 21 out of 50 samples in Group A were found positive for . AP-PCR showed nine different genotypes in Group B and six different genotypes in Group A. Heteroduplex-PCR showed a total of seven different genotypes in Group B and five different genotypes in Group A.
Results of this study show that, in diabetic condition, shows increased variations in genotypes.
糖尿病已成为全球的一种流行病。牙周炎与糖尿病相关，并且它已被视为糖尿病的第六种并发症。然而，糖尿病对诸如牙周病致病微生物群的作用仍不清楚。
通过使用任意引物聚合酶链反应（AP-PCR）和异源双链PCR，比较有糖尿病和无糖尿病的慢性牙周炎患者中不同基因型的总数。
这是一项单中心比较研究。
本研究共纳入100例患者。患者分为两组：A组——患有慢性牙周炎但无糖尿病的患者（50例）和B组——患有慢性牙周炎且有糖尿病的患者（50例）。从两组患者中采集龈下菌斑样本。菌斑样本培养……收集阳性培养样本和通过蛋白酶K法从样本中提取的DNA。部分DNA样本使用OPA-13引物通过AP-PCR进行检测，部分DNA使用特异性Pg8引物通过异源双链PCR进行检测。结果通过聚丙烯酰胺凝胶电泳程序进行分析。
使用独立t检验和卡方检验（SPSS 16）。
B组50个样本中有34个检测到……呈阳性，而A组50个样本中有21个检测到……呈阳性。AP-PCR显示B组有9种不同基因型，A组有6种不同基因型。异源双链PCR显示B组共有7种不同基因型，A组有5种不同基因型。
本研究结果表明，在糖尿病状态下，……的基因型变异增加。
