Padmalatha G V, Bavle Radhika M, Satyakiran Gadavalli Vera Venkata, Paremala K, Sudhakara M, Makarla Soumya
Department of Oral and Maxillofacial Pathology, Krishnadevaraya College of Dental Sciences, Bengaluru, Karnataka, India.
J Oral Maxillofac Pathol. 2016 Sep-Dec;20(3):413-418. doi: 10.4103/0973-029X.190933.
Periodontal diseases, if left untreated, can lead to tooth loss and affect at least one tooth in 80% of adults worldwide, with the main cause being a bacterial plaque. Among subgingival plaque bacterial species, has been implicated as a major etiological agent causing tooth loss. Diabetics and smokers are two patient groups at high risk for periodontal disease. The increase in the number of this organism with the coexistence of other pathogenic microbes leads to rapid destruction of the periodontium, premature loss of teeth and also because of its virulence has implications in systemic pathology. Our aim was to observe the involvement of in diabetes mellitus (DM) patients associated with periodontitis with and without tobacco-associated habits and to compare them with periodontitis patients having no other systemic pathologies.
Subgingival plaque samples from a total of seventy subjects were included in the study. DNA was isolated from the collected sample and was quantified using spectrophotometer for standardizing the polymerase chain reaction. The quantity of the isolated DNA was checked in a ultraviolet-visible spectrophotomer.
One-way ANOVA and Tukey's multiple procedures were carried out.
The maximum score of was seen in periodontitis patients having DM, whereas the least score was seen in periodontitis patients having DM with tobacco smoking habit compared to the other groups.
count is significantly reduced in periodontitis patients having DM with smoking habit; it is concluded that might not be a key causative organism responsible for the periodontal destruction in case of smokers despite the DM condition. The decrease in counts may be attributed to change in the local environment like chemical (tobacco nitrosamines) and physical changes preventing the growth of .
牙周疾病若不治疗，会导致牙齿脱落，全球80%的成年人至少有一颗牙齿受影响，主要病因是细菌菌斑。在龈下菌斑细菌种类中，[某种细菌]被认为是导致牙齿脱落的主要病原体。糖尿病患者和吸烟者是牙周疾病的两个高危患者群体。这种微生物数量的增加与其他致病微生物共存，会导致牙周组织迅速破坏、牙齿过早脱落，而且由于其毒性，还会对全身病理产生影响。我们的目的是观察[某种细菌]在患有和未患有与烟草相关习惯的牙周炎糖尿病（DM）患者中的情况，并将他们与没有其他全身病理状况的牙周炎患者进行比较。
本研究共纳入70名受试者的龈下菌斑样本。从收集的样本中分离出DNA，并使用分光光度计进行定量，以标准化聚合酶链反应。在紫外可见分光光度计中检查分离出的DNA的量。
进行单因素方差分析和Tukey多重比较程序。
在患有糖尿病的牙周炎患者中，[某种细菌]的得分最高，而与其他组相比，在患有糖尿病且有吸烟习惯的牙周炎患者中得分最低。
在患有糖尿病且有吸烟习惯的牙周炎患者中，[某种细菌]的数量显著减少；得出的结论是，尽管存在糖尿病状况，但对于吸烟者而言，[某种细菌]可能不是导致牙周破坏的关键病原体。数量的减少可能归因于局部环境的变化，如化学变化（烟草亚硝胺）和物理变化，从而阻止了[某种细菌]的生长。
