Lalla Evanthia, Kaplan Selma, Chang Shu-mei J, Roth Georg A, Celenti Romanita, Hinckley Karen, Greenberg Ellen, Papapanou Panos N
Division of Periodontics, Section of Oral and Diagnostic Sciences, College of Dental Medicine, Columbia University, New York, NY 10032, USA.
J Clin Periodontol. 2006 Dec;33(12):855-62. doi: 10.1111/j.1600-051X.2006.00996.x.
We investigated the levels of subgingival plaque bacteria and serum IgG responses in patients with type 1 diabetes and non-diabetic controls of comparable periodontal status.
Fifty type 1 diabetes patients (mean duration 20.3 years, range 6-41) were age-and gender-matched with 50 non-diabetic individuals with similar levels of periodontal disease. Full-mouth clinical periodontal status was recorded, and eight plaque samples/person were collected and analysed by checkerboard hybridization with respect to 12 species. Homologous serum IgG titres were assessed by checkerboard immunoblotting. In a sub-sample of pairs, serum cytokines and selected markers of cardiovascular risk were assessed using multiplex technology.
Among the investigated species, only levels of Eubacterium nodatum were found to be higher in diabetic patients, while none of the IgG titres differed between the groups, both before and after adjustments for microbial load. Patients with diabetes had significantly higher serum levels of soluble E-selectin (p=0.04), vascular cell adhesion molecule-1 (VCAM-1; p=0.0008), adiponectin (p=0.01) and lower levels of plasminogen activator inhibitor-1 (PAI-1; p=0.02).
After controlling for the severity of periodontal disease, patients with type 1 diabetes and non-diabetic controls showed comparable subgingival infection patterns and serum antibody responses.
我们调查了1型糖尿病患者和牙周状况相当的非糖尿病对照者的龈下菌斑细菌水平和血清IgG反应。
50例1型糖尿病患者（平均病程20.3年，范围6 - 41年）与50例牙周疾病水平相似的非糖尿病个体进行年龄和性别匹配。记录全口临床牙周状况，每人收集8份菌斑样本，通过棋盘杂交法分析12种细菌。通过棋盘免疫印迹法评估同源血清IgG滴度。在配对的子样本中，使用多重技术评估血清细胞因子和选定的心血管风险标志物。
在研究的细菌种类中，仅发现糖尿病患者中诺氏真杆菌水平较高，而在调整微生物负荷前后，两组之间的IgG滴度均无差异。糖尿病患者的血清可溶性E选择素（p = 0.04）、血管细胞黏附分子-1（VCAM-1；p = 0.0008）、脂联素（p = 0.01）水平显著升高，纤溶酶原激活物抑制剂-1（PAI-1；p = 0.02）水平降低。
在控制牙周疾病严重程度后，1型糖尿病患者和非糖尿病对照者表现出相当的龈下感染模式和血清抗体反应。
