De Carolis Elena, Soldini Silvia, La Rosa Marilisa, Nucci Fabio, Posteraro Brunella, Sanguinetti Maurizio
Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy.
Dipartimento di Scienze Gastroenterologiche, Endocrino-Metaboliche e Nefro-Urologiche, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy.
Front Microbiol. 2019 Aug 30;10:2046. doi: 10.3389/fmicb.2019.02046. eCollection 2019.
is the most frequent cause of catheter-related candidemia among non- species. This may be related to intrinsic capabilities as adhering and forming a biofilm on abiotic surfaces such as on medical devices. As previously demonstrated, patients infected with high biofilm-producing isolates had a greater mortality risk compared to patients infected with low biofilm-producing isolates. We developed the BIOF-HILO assay, a MALDI-TOF mass spectrometry (MS)-based assay, which compares mass spectra obtained from attached and suspended isolate cells during the early (i.e., 3-h) adhesion phase of biofilm formation. The composite correlation index (CCI) analysis was used to discriminate between mass spectra differences of the two cell types, classifying all 50 clinical isolates, included in the study, after only 3-h of testing, in high or low biofilm producers. All high ( = 25) or low ( = 25) biofilm producers had, according to CCI mass spectra comparison values, higher or lower than one CCI ratios, which were obtained by dividing the CCI by the CCI. In conclusion, the BIOF-HILO assay allows a rapid categorization of clinical isolates in high or low biofilm producers. This information, if timely provided to physicians, may improve treatment outcomes in patients with candidemia.
是非念珠菌属中导管相关念珠菌血症最常见的病因。这可能与在诸如医疗设备等非生物表面上粘附并形成生物膜的内在能力有关。如先前所示，与感染低生物膜产生型菌株的患者相比，感染高生物膜产生型菌株的患者有更高的死亡风险。我们开发了BIOF-HILO检测法，这是一种基于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的检测法，可比较在生物膜形成早期（即3小时）粘附阶段从附着和悬浮的分离细胞获得的质谱。复合相关指数（CCI）分析用于区分两种细胞类型的质谱差异，在仅3小时的检测后，将研究中纳入的所有50株临床分离株分类为高生物膜产生者或低生物膜产生者。根据CCI质谱比较值，所有高生物膜产生者（n = 25）或低生物膜产生者（n = 25）的CCI比率高于或低于一个通过将CCI除以CCI获得的比率。总之，BIOF-HILO检测法可快速将临床分离株分类为高生物膜产生者或低生物膜产生者。如果及时向医生提供此信息，可能会改善念珠菌血症患者的治疗结果。
