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犬胎儿肾发生区肾单位祖细胞及其早期上皮衍生结构的分子特征。

Molecular characterization of nephron progenitors and their early epithelial derivative structures in the nephrogenic zone of the canine fetal kidney.

机构信息

Department of Biomedical Sciences, College of Veterinary Medicine, Tuskegee University, Tuskegee.

出版信息

Eur J Histochem. 2019 Sep 23;63(3):3049. doi: 10.4081/ejh.2019.3049.

DOI:10.4081/ejh.2019.3049
PMID:31544449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6763752/
Abstract

Nephron progenitors (NPs) and nephrogenesis have been extensively studied in mice and humans and have provided insights into the mechanisms of renal development, disease and possibility of NP-based therapies. However, molecular features of NPs and their derivatives in the canine fetal kidney (CFK) remain unknown. This study was focused to characterize the expression of potential markers of canine NPs and their derivatives by immuno-fluorescence and western blot analysis. Transcription factors (TFs) SIX1 and SIX2, well-characterized human NP markers, were expressed in NPs surrounding the ureteric bud in the CFK. Canine NPs also expressed ITGA8 and NCAM1, surface markers previously used to isolate NPs from the mouse and human fetal kidneys. TF, PAX2 was detected in the ureteric bud, NPs and their derivative structures such as renal vesicle and S-shaped body. This study highlights the similarities in dog, mouse and human renal development and characterizes markers to identify canine NPs and their derivatives. These results will facilitate the isolation of canine NPs and their functional characterization to develop NP-based therapies for canine renal diseases.

摘要

肾祖细胞 (NPs) 和肾发生在小鼠和人类中得到了广泛研究，为肾脏发育、疾病的机制和基于 NPs 的治疗的可能性提供了深入的了解。然而，犬胎儿肾脏 (CFK) 中 NPs 及其衍生物的分子特征仍不清楚。本研究旨在通过免疫荧光和 Western blot 分析来表征犬 NPs 及其衍生物的潜在标志物的表达。转录因子 (TFs) SIX1 和 SIX2 是人类 NPs 的特征标志物，在 CFK 中围绕输尿管芽表达。犬 NPs 还表达了 ITGA8 和 NCAM1，这是以前用于从鼠和人胎儿肾脏中分离 NPs 的表面标志物。TF、PAX2 被检测到在输尿管芽、NPs 及其衍生物结构如肾小囊和 S 形体中。本研究强调了狗、老鼠和人类肾脏发育的相似性，并描述了用于鉴定犬 NPs 及其衍生物的标志物。这些结果将有助于分离犬 NPs 及其功能特征化，为犬肾脏疾病开发基于 NPs 的治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b93/6763752/cd5a245dad0b/ejh-63-3-3049-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b93/6763752/73f577b1e4c1/ejh-63-3-3049-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b93/6763752/cd5a245dad0b/ejh-63-3-3049-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b93/6763752/5220e3c3ab65/ejh-63-3-3049-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b93/6763752/34d30557fd82/ejh-63-3-3049-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b93/6763752/46f1f4e76ab9/ejh-63-3-3049-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b93/6763752/3e777ae99a1d/ejh-63-3-3049-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b93/6763752/73f577b1e4c1/ejh-63-3-3049-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b93/6763752/cd5a245dad0b/ejh-63-3-3049-g007.jpg

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Annu Rev Physiol. 2019 Feb 10;81:335-357. doi: 10.1146/annurev-physiol-020518-114331.
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Dissecting the Global Dynamic Molecular Profiles of Human Fetal Kidney Development by Single-Cell RNA Sequencing.通过单细胞 RNA 测序解析人类胎儿肾脏发育的全球动态分子图谱。
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