Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Phytopathology. 2020 Jan;110(1):153-163. doi: 10.1094/PHYTO-06-19-0199-FI. Epub 2019 Nov 29.
(TSWV) is a negative-stranded RNA virus that infects hundreds of plant species, causing great economic loss. Infected plants develop symptoms including chlorosis and wilt, which can lead to cell death. From 9 to 15 days after TSWV infection, symptoms progress through a three-stage process of appearance, severity, and death. In this study, deep sequencing technology was first used to explore gene expression in response to TSWV infection in model plant at different symptom development stages. We found that plant immune defense and protein degradation are induced by TSWV infection and that both inductions became stronger over time. The photosynthesis pathway was attenuated with TSWV infection. Cell wall metabolism had a large extent of downregulation while some genes were upregulated. These results illustrate the dynamic nature of TSWV infection in . at the whole-transcriptome level. The link between biological processes and subpathway metabolism was further analyzed. Our study provides new insight into host regulatory networks and dynamic processes in response to TSWV infection.
(TSWV)是一种负链 RNA 病毒，可感染数百种植物物种，造成巨大的经济损失。受感染的植物会出现黄化和萎蔫等症状，这可能导致细胞死亡。在 TSWV 感染后的 9 到 15 天内，症状会经历外观、严重程度和死亡三个阶段的进展。在这项研究中，我们首次使用深度测序技术在不同症状发展阶段的模式植物中探索了对 TSWV 感染的基因表达。我们发现，植物的免疫防御和蛋白质降解被 TSWV 感染诱导，并且随着时间的推移，这两种诱导作用变得更强。光合作用途径在 TSWV 感染时受到抑制，细胞壁代谢在很大程度上受到下调，而一些基因则被上调。这些结果说明了 TSWV 在 中的感染在整个转录组水平上的动态性质。还进一步分析了生物过程和亚途径代谢之间的联系。我们的研究为宿主调控网络和对 TSWV 感染的动态过程提供了新的见解。
