Hu Dongxin, Zhang Mingyan, Peng Zhongmin
Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.
Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.
J Cancer Res Ther. 2020 Sep;16(5):1157-1164. doi: 10.4103/jcrt.JCRT_953_20.
As one of the most common malignant tumors of the digestive tract, esophageal squamous cell carcinoma (ESCC) is an advanced metastatic cancer with an extremely high mortality rate and the highest prevalence rate in China. Spindle- and kinetochore-associated protein 1 (SKA1), an essential member involved in chromosome separation during mitosis, has been indicated as a potential biomarker in the pathogenesis and development of various types of malignant tumors; however, the exact functions of SKA1 in ESCC are still unclear.
SKA1 expression was explored in stage IIA ESCC and corresponding healthy esophageal mucosa tissues through immunohistochemistry and reverse transcription-quantitative polymerase chain reaction and was further validated using The Cancer Genome Atlas (TCGA) database of the online tool UALCAN. Then, the clinicopathological correlations of SKA1 were analyzed based on the follow-up data. Furthermore, using the online tool LinkedOmics, the correlation test, gene ontology (GO), and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of SKA1 were analyzed using high-throughput sequencing data of ESCC patients from TCGA dataset.
The expression level of SKA1 was markedly upregulated in ESCC tissues. Upregulation of SKA1 significantly correlated with higher pathological T stage (P = 0.003) and poorer overall survival (P = 0.013). GO and pathway enrichment analyses of SKA1 in ESCC revealed that SKA1 was involved in a number of classical cell cycle-related pathways that contribute to special biological processes in tumorigenesis and development of ESCC.
The results of this study demonstrate that SKA1 may act as a prognostic biomarker for stage IIA ESCC. Combined with the bioinformatic analysis, SKA1 could potentially serve as a therapeutic target for ESCC.
The results coming from the present study demonstrated that SKA1 may act as a prognostic biomarker for stage IIA ESCC. Combined with the bioinformatic analysis, SKA1 could serve as a potential therapeutic target for ESCC.
食管鳞状细胞癌（ESCC）作为消化道最常见的恶性肿瘤之一，是一种晚期转移性癌症，死亡率极高，在中国的发病率也最高。纺锤体和动粒相关蛋白1（SKA1）是有丝分裂过程中参与染色体分离的重要成员，已被指出是各类恶性肿瘤发病机制和发展过程中的潜在生物标志物；然而，SKA1在ESCC中的具体功能仍不清楚。
通过免疫组织化学和逆转录定量聚合酶链反应，在IIA期ESCC及相应的健康食管黏膜组织中探究SKA1表达，并使用在线工具UALCAN的癌症基因组图谱（TCGA）数据库进行进一步验证。然后，根据随访数据分析SKA1的临床病理相关性。此外，使用在线工具LinkedOmics，利用来自TCGA数据集的ESCC患者高通量测序数据，对SKA1进行相关性检验、基因本体（GO）和京都基因与基因组百科全书通路富集分析。
ESCC组织中SKA1的表达水平显著上调。SKA1的上调与更高的病理T分期（P = 0.003）和更差的总生存期（P = 0.013）显著相关。对ESCC中SKA1的GO和通路富集分析表明，SKA1参与了许多经典的细胞周期相关通路，这些通路有助于ESCC肿瘤发生和发展中的特殊生物学过程。
本研究结果表明，SKA1可能作为IIA期ESCC的预后生物标志物。结合生物信息学分析，SKA1可能成为ESCC的治疗靶点。
本研究结果表明，SKA1可能作为IIA期ESCC的预后生物标志物。结合生物信息学分析，SKA1可作为ESCC的潜在治疗靶点。
