WPI-MANA, National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan.
Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Mater Sci Eng C Mater Biol Appl. 2019 Dec;105:110108. doi: 10.1016/j.msec.2019.110108. Epub 2019 Aug 21.
Hydrophilic epoxy resin-based monoliths were employed as cell culture substrates. The monoliths were made of a porous material with a bicontinuous structure that consisted of a porous channel and a resin skeleton. Monolith disks were prepared with a skinless surface through polymerization-induced spinodal decomposition-type phase separation. The pore sizes, which were well controlled by the polymerization temperature, ranged from 70 to 380 nm. The quantity of protein adsorbed per unit area and the early-stage adhesion of HepG2 cells on the monolith substrates were independent of pore size, meaning they were not affected by surface topology. Long-term cell adhesion, as indicated by adherent cell number and shape, as well as liver-specific gene expression were significantly affected by pore size. In terms of cell shape, number, and gene expression, pores of approximately 200 nm were most suitable for HepG2 cell growth. These results highlight the importance of monolith morphology for use as a cell culture substrate. The well-controlled morphology demonstrated in this work indicates monoliths are capable of supporting growth for various types of cells in a range of applications.
水凝胶环氧树脂基整体材料被用作细胞培养基质。整体材料由多孔材料制成，具有双连续结构，由多孔通道和树脂骨架组成。通过聚合诱导的旋节分解型相分离制备了具有无表皮表面的整体材料盘。聚合温度可以很好地控制孔径，范围从 70 到 380nm。单位面积吸附的蛋白质量和 HepG2 细胞在整体材料基质上的早期粘附与孔径无关，这意味着它们不受表面拓扑结构的影响。长期细胞粘附，表现为附着细胞的数量和形状以及肝脏特异性基因表达，显著受到孔径的影响。就细胞形状、数量和基因表达而言，孔径约为 200nm 的孔最适合 HepG2 细胞生长。这些结果强调了整体材料形态用作细胞培养基质的重要性。本工作中展示的良好控制的形态表明，整体材料能够在各种应用中支持多种类型细胞的生长。
