Bioscience and Textile Technology Department, Shinshu University, Ueda, Nagano, Japan; Department of Textile Engineering, Mehran University of Engineering and Technology, Jamshoro 76062, Pakistan.
Nano Fusion Technology Research Group, Division of Frontier Fibers, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Tokida 3-15-1, Ueda, Nagano Prefecture 386-8567, Japan.
Mater Sci Eng C Mater Biol Appl. 2019 Dec;105:110077. doi: 10.1016/j.msec.2019.110077. Epub 2019 Aug 12.
Although silver based nanofibers possess excellent bactericidal and bacteriostatic characteristics. However, excess release/contact with silver may induce harmful side-effects including carcinoma, argyria, argyrosis and allergies. Similarly, silver depletion may limit prolonged antibacterial activities as well. Thus present research proposes electrospun CA/ZnO/AgNPs composite nanofibers for biologically safer and sustained antibacterial applications. The ZnO/AgNPs were synthesized using dopamine hydrochloride (Dopa) as reducing agent to immobilize AgNPs on ZnO nanoparticles. A simple solution-mixing procedure effectively generated AgNPs on ZnO nanoparticles. Strong adhesive characteristics of Dopa initiate adsorption of silver ions on ZnO nanoparticle surfaces and its metal ion reducing properties generate AgNPs. Additionally, the Dopa mediation generates strongly adhered AgNPs. The ZnO/AgNPs were used to fabricate CA/ZnO/AgNPs nanofibers. Characterization techniques, XRD, XPS, TEM, FTIR and SEM confirmed synthesis of nanocomposites. Crystallite sizes of ZnO and AgNPs calculated by Debye-Scherrer equation were 17.85 nm and 11.68 nm respectively. Antibacterial assays confirmed CA/ZnO/AgNP's effectiveness in growth inhibition of E. coli and S. aureus strains on agar plate and in liquid medium. The nanofiber composites demonstrated 100% bactericidal properties against both the test strains. Bacterial growth inhibition in LB medium for 108 h indicated suitability of CA/ZnO/AgNPs composite nanofibers in sustained antibacterial applications such as antibacterial wound dressings and other applications demanding sustained antimicrobial properties.
尽管基于银的纳米纤维具有优异的杀菌和抑菌特性。然而，过量的银释放/接触可能会引起有害的副作用，包括癌、银沉着症、银中毒和过敏。同样，银的耗尽也可能限制其长期的抗菌活性。因此，本研究提出了电纺 CA/ZnO/AgNPs 复合纳米纤维，用于更安全和持续的抗菌应用。使用多巴胺盐酸盐（Dopa）作为还原剂，将 ZnO/AgNPs 合成，将 AgNPs 固定在 ZnO 纳米颗粒上。一种简单的溶液混合程序有效地在 ZnO 纳米颗粒上生成了 AgNPs。Dopa 的强粘附特性引发了银离子在 ZnO 纳米颗粒表面的吸附，其金属离子还原特性生成了 AgNPs。此外，Dopa 介导生成了强粘附的 AgNPs。使用 ZnO/AgNPs 制备了 CA/ZnO/AgNPs 纳米纤维。XRD、XPS、TEM、FTIR 和 SEM 等表征技术证实了纳米复合材料的合成。通过德拜-谢勒方程计算的 ZnO 和 AgNPs 的晶粒尺寸分别为 17.85nm 和 11.68nm。抗菌试验证实了 CA/ZnO/AgNP 在琼脂平板和液体培养基中对大肠杆菌和金黄色葡萄球菌的生长抑制的有效性。纳米纤维复合材料对两种测试菌株均表现出 100%的杀菌性能。在 LB 培养基中 108 小时的细菌生长抑制表明 CA/ZnO/AgNPs 复合纳米纤维在持续抗菌应用中的适用性，如抗菌伤口敷料和其他需要持续抗菌性能的应用。
