鱼油而非橄榄油可改善慢性轻度应激大鼠的抑郁样行为和肠道微生物失调。

Fish Oil, but Not Olive Oil, Ameliorates Depressive-Like Behavior and Gut Microbiota Dysbiosis in Rats under Chronic Mild Stress.

机构信息

School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan.

Graduate Institute of Metabolism and Obesity Sciences, Taipei Medical University, Taipei 11031, Taiwan.

出版信息

Biomolecules. 2019 Sep 21;9(10):516. doi: 10.3390/biom9100516.

BACKGROUND

This study investigated the effects of fish oil and olive oil in improving dysbiosis and depressive-like symptoms.

METHODS AND RESULTS

Male rats were fed normal, fish oil-rich or olive oil-rich diets for 14 weeks. Chronic mild stress (CMS) was administered from week 2. The sucrose preference test (SPT) and forced swimming test (FST) were used to determine depressive-like behavior. The SPT results revealed that the CMS, CMS with imipramine (CMS+P) treatment, and CMS with olive oil diet (CMS+O) groups exhibited significantly reduced sucrose intake from week 8, whereas the fish oil diet (CMS+F) group exhibited significantly reduced sucrose intake from week 10. The FST results showed that the immobile time of the CMS+F group was significantly less than that of the CMS-only group. Next generation sequencing (NGS) results showed CMS significantly reduced the abundance of and increased that of and . However, the CMS+F group showed an increase in the abundance of , , and , whereas the CMS+O group showed an increase in the abundance of .

CONCLUSIONS

CMS stimuli altered the gut microbiome in depressed rats. Fish oil and olive oil exerted part of a prebiotic-like effect to ameliorate dysbiosis induced by CMS. However, only fish oil ameliorated depressive-like symptoms.

背景

本研究旨在探讨鱼油和橄榄油对改善肠道菌群失调和抑郁样症状的影响。

方法和结果

雄性大鼠连续 14 周分别给予正常饮食、富含鱼油饮食或富含橄榄油饮食，从第 2 周开始给予慢性轻度应激（CMS）。采用蔗糖偏好试验（SPT）和强迫游泳试验（FST）检测大鼠的抑郁样行为。SPT 结果显示，CMS 组、CMS 联合丙咪嗪（CMS+P）治疗组和 CMS 联合橄榄油饮食（CMS+O）组从第 8 周开始蔗糖摄入量显著减少，而 CMS 联合鱼油饮食（CMS+F）组从第 10 周开始蔗糖摄入量显著减少。FST 结果显示，CMS+F 组大鼠的不动时间显著少于 CMS 组。下一代测序（NGS）结果显示，CMS 显著降低了和的丰度，增加了和的丰度。然而，CMS+F 组大鼠的、、和的丰度增加，而 CMS+O 组大鼠的、、和的丰度增加。