Translational Psychiatry Laboratory, Graduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, Brazil.
Translational Psychiatry Laboratory, Graduate Program in Health Sciences, Health Sciences Unit, University of Southern Santa Catarina, Criciúma, Brazil.
Nutrition. 2018 Sep;53:120-133. doi: 10.1016/j.nut.2018.03.006. Epub 2018 Mar 30.
To investigate the antidepressant and antioxidant effects of omega-3, folic acid and n-acetylcysteine (NAC) in rats which were subjected to early or late life stress.
Early stress was induced through maternal deprivation (MD), while late life stress was induced using the chronic mild stress (CMS) protocol. Young rats which were subjected to MD and the adult rats which were subjected to CMS were treated with omega-3 fatty acids (0.72 g/kg), NAC (20 mg/kg) or folic acid (50 mg/kg) once/day, for a period of 20 days. Then, the animals' immobility times were evaluated using the forced swimming test. Oxidative stress parameters were evaluated in the brain.
Depressive-like behavior induced by CMS was prevented by NAC and folic acid, and depressive-like behavior induced by MD was prevented by NAC, folic acid and omega-3. NAC, folic acid and omega-3 were able to exert antioxidant effects in the brain of rats subjected to CMS or MD. These preventive treatments decreased the levels of protein carbonylation and lipid peroxidation, and also decreased the concentrations of nitrite/nitrate and reduced the activity of myeloperoxidase activity in the rat brain which was induced by CMS or MD. NAC, folic acid and omega-3 increased superoxide dismutase and catalase activities in the rat brain subjected to early or late life stress.
NAC, omega-3 and folic acid may present interesting lines of treatment based on their antioxidant properties, which cause an inhibition of behavioral and brain changes that occur from stressful life events.
研究ω-3、叶酸和 N-乙酰半胱氨酸（NAC）对经历早期或晚期生活应激的大鼠的抗抑郁和抗氧化作用。
通过母婴分离（MD）诱导早期应激，通过慢性轻度应激（CMS）方案诱导晚期生活应激。接受 MD 的幼鼠和接受 CMS 的成年大鼠每天接受一次 ω-3 脂肪酸（0.72g/kg）、NAC（20mg/kg）或叶酸（50mg/kg）治疗，共 20 天。然后，使用强迫游泳试验评估动物的不动时间。评估大脑中的氧化应激参数。
CMS 诱导的抑郁样行为被 NAC 和叶酸预防，MD 诱导的抑郁样行为被 NAC、叶酸和 ω-3 预防。NAC、叶酸和 ω-3 能够在 CMS 或 MD 大鼠的大脑中发挥抗氧化作用。这些预防治疗降低了 CMS 或 MD 诱导的大鼠大脑中蛋白质羰基化和脂质过氧化水平，降低了亚硝酸盐/硝酸盐浓度，并降低了 CMS 或 MD 诱导的大鼠大脑中髓过氧化物酶活性。NAC、叶酸和 ω-3 增加了早期或晚期生活应激大鼠大脑中超氧化物歧化酶和过氧化氢酶的活性。
NAC、ω-3 和叶酸可能因其抗氧化特性而具有有趣的治疗方法，可抑制应激性生活事件引起的行为和大脑变化。
