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岩藻黄质 A 促进骨髓间充质干细胞成骨向分化

Phlorofucofuroeckol A from Edible Brown Alga Enhances Osteoblastogenesis in Bone Marrow-Derived Human Mesenchymal Stem Cells.

机构信息

Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Busan 46958, Korea.

Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University, Busan 49112, Korea.

出版信息

Mar Drugs. 2019 Sep 21;17(10):543. doi: 10.3390/md17100543.

DOI:10.3390/md17100543
PMID:31546680
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6836260/
Abstract

The deterioration of bone formation is a leading cause of age-related bone disorders. Lack of bone formation is induced by decreased osteoblastogenesis. In this study, osteoblastogenesis promoting effects of algal phlorotannin, phlorofucofuroeckol A (PFF-A), were evaluated. PFF-A was isolated from brown alga . The ability of PFF-A to enhance osteoblast differentiation was observed in murine pre-osteoblast cell line MC3T3-E1 and human bone marrow-derived mesenchymal stem cells (huBM-MSCs). Proliferation and alkaline phosphatase (ALP) activity of osteoblasts during differentiation was assayed following PFF-A treatment along extracellular mineralization. In addition, effect of PFF-A on osteoblast maturation pathways such as Runx2 and Smads was analyzed. Treatment of PFF-A was able to enhance the proliferation of differentiating osteoblasts. Also, ALP activity was observed to be increased. Osteoblasts showed increased extracellular mineralization, observed by Alizarin Red staining, following PFF-A treatment. In addition, expression levels of critical proteins in osteoblastogenesis such as ALP, bone morphogenetic protein-2 (BMP-2), osteocalcin and β-catenin were stimulated after the introduction of PFF-A. In conclusion, PFF-A was suggested to be a potential natural product with osteoblastogenesis enhancing effects which can be utilized against bone-remodeling imbalances and osteoporosis-related complications.

摘要

骨形成的恶化是与年龄相关的骨骼疾病的主要原因。骨形成的减少是由成骨细胞生成减少引起的。在这项研究中，评估了藻类岩藻多酚、岩藻黄素 A（PFF-A）对成骨细胞生成的促进作用。PFF-A 是从褐藻中分离出来的。观察了 PFF-A 在小鼠前成骨细胞系 MC3T3-E1 和人骨髓间充质干细胞（huBM-MSCs）中增强成骨细胞分化的能力。在用 PFF-A 处理后，检测了成骨细胞分化过程中细胞增殖和碱性磷酸酶（ALP）活性以及细胞外矿化。此外，还分析了 PFF-A 对成骨细胞成熟途径（如 Runx2 和 Smads）的影响。PFF-A 的处理能够增强分化中的成骨细胞的增殖。同时，观察到 ALP 活性增加。用 PFF-A 处理后，成骨细胞的细胞外矿化增加，茜素红染色可见。此外，在引入 PFF-A 后，成骨细胞生成中关键蛋白的表达水平，如 ALP、骨形态发生蛋白-2（BMP-2）、骨钙素和 β-连环蛋白，也得到了刺激。总之，PFF-A 被认为是一种具有成骨细胞生成增强作用的潜在天然产物，可用于治疗骨骼重塑失衡和与骨质疏松症相关的并发症。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/46f25bc70998/marinedrugs-17-00543-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/b90629b33f19/marinedrugs-17-00543-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/dac0bc3bfda4/marinedrugs-17-00543-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/015f60fa55aa/marinedrugs-17-00543-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/79a0e6f84577/marinedrugs-17-00543-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/c79852fe9e7e/marinedrugs-17-00543-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/b4c3964181c7/marinedrugs-17-00543-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/46f25bc70998/marinedrugs-17-00543-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/b90629b33f19/marinedrugs-17-00543-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/dac0bc3bfda4/marinedrugs-17-00543-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/015f60fa55aa/marinedrugs-17-00543-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/79a0e6f84577/marinedrugs-17-00543-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/c79852fe9e7e/marinedrugs-17-00543-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/b4c3964181c7/marinedrugs-17-00543-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d13/6836260/46f25bc70998/marinedrugs-17-00543-g007.jpg

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