Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Nutrients. 2019 Sep 19;11(9):2254. doi: 10.3390/nu11092254.
Ovotransferrin, a member of the transferrin family, is the second main protein found in egg white. Ovotransferrin was reported to have antimicrobial, antioxidant, and immunomodulating activities. The aim of this work was to characterize the cellular and molecular functions of egg white ovotransferrin on osteoclasts differentiation and function. Osteoclasts were prepared from mouse macrophage RAW 264.7 cells stimulated with receptor activator of nuclear factor κB ligand (RANKL). Ovotransferrin inhibited osteoclasts differentiation and the calcium-phosphate resorptive ability via the suppression of RANKL-induced nuclear factor κ-light chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Ovotransferrin induced apoptosis of matured osteoclasts, accompanied by increased expression of Bcl-2-like protein 11 () and Bcl-2-assoicated death promoter (), but decreased expression of B-cell lymphoma 2 () and B-cell lymphoma-extra-large (). We established a novel role of egg white ovotransferrin as an inhibitor of osteoclastogenesis, which may be used for the prevention of osteoporosis.
卵转铁蛋白是转铁蛋白家族的一员，是蛋清中发现的第二大主要蛋白质。卵转铁蛋白具有抗菌、抗氧化和免疫调节活性。本工作旨在研究蛋清卵转铁蛋白对破骨细胞分化和功能的细胞和分子功能。破骨细胞是从小鼠巨噬细胞 RAW 264.7 细胞中用核因子κB 受体激活剂配体（RANKL）刺激制备的。卵转铁蛋白通过抑制 RANKL 诱导的核因子κB 轻链增强子的激活 B 细胞（NF-κB）和丝裂原激活蛋白激酶（MAPK）信号通路来抑制破骨细胞的分化和钙磷吸收能力。卵转铁蛋白诱导成熟破骨细胞凋亡，伴随 Bcl-2 样蛋白 11（）和 Bcl-2 相关死亡启动子（）表达增加，而 B 细胞淋巴瘤 2（）和 B 细胞淋巴瘤-extra-large（）表达减少。我们确立了蛋清卵转铁蛋白作为破骨细胞生成抑制剂的新作用，可用于预防骨质疏松症。
