Bernardi Tatiana, Bortolini Olga, Massi Alessandro, Sacchetti Gianni, Tacchini Massimo, De Risi Carmela
Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Ferrara, Via Luigi Borsari 46, I-44121 Ferrara, Italy.
Dipartimento di Scienze della Vita e Biotecnologie, Sezione di Botanica Applicata, Piazzale Luciano Chiappini 2, Malborghetto di Boara, I-44123 Ferrara, Italy.
Molecules. 2019 Sep 20;24(19):3416. doi: 10.3390/molecules24193416.
Both environmental and economic issues are increasingly pushing for the revalorization of agri-food by-products, including those arising from wine industry. Wastes produced from wine-making processes are important sources of biologically active compounds, mainly phenolic acids and flavonoids, which could be re-used for several applications, for example as additive surrogates or new ingredients in foodstuffs and/or pharmaceuticals. Therefore, the development of methods aimed at isolating, characterizing and quantifying molecules present in winery by-products acquires considerable importance in view of their re-utilization on a large scale. In this connection, this study demonstrated that high-performance thin-layer chromatography (HPTLC) and high-performance liquid chromatography with diode array detection (HPLC-DAD) can operate in synergy for the investigation of pomace and seed materials arising from both white and red cultivars of . By virtue of fingerprint profiling, mass spectrometry (MS) interfacing and band comparison method, HPTLC enabled detection and identification of phenolic acids, non-anthocyanic flavonoids and anthocyanins. On the contrary, only anthocyanins could be identified by HPLC-DAD, and their subsequent quantification showed that malvidin-3--glucoside (oenin) was the most abundant one. In parallel, HPTLC has allowed to detect and quantify proanthocyanidins (PAC), showing that only catechin was present in the test samples. Both quantitative analytical methods were validated in terms of linearity, detection and quantification limits and precision.
环境和经济问题都在日益推动对农业食品副产品进行增值利用，包括葡萄酒行业产生的副产品。酿酒过程产生的废弃物是生物活性化合物的重要来源，主要是酚酸和黄酮类化合物，这些化合物可重新用于多种用途，例如作为食品和/或药品中的添加剂替代物或新成分。因此，鉴于酿酒厂副产品的大规模再利用，开发旨在分离、表征和定量其中存在的分子的方法具有相当重要的意义。在这方面，本研究表明，高效薄层色谱法（HPTLC）和带二极管阵列检测的高效液相色谱法（HPLC-DAD）可以协同操作，用于研究白葡萄和红葡萄品种产生的果渣和种子材料。借助指纹图谱分析、质谱（MS）联用和条带比较法，HPTLC能够检测和鉴定酚酸、非花青素类黄酮和花青素。相反，HPLC-DAD只能鉴定花青素，其后续定量分析表明，矢车菊素-3-葡萄糖苷（oenin）是含量最丰富的一种。同时，HPTLC还能够检测和定量原花青素（PAC），结果表明测试样品中仅存在儿茶素。两种定量分析方法均在线性、检测限和定量限以及精密度方面进行了验证。
