Faculty of Health Sciences, 70987University of Beira Interior, Covilhã, Portugal.
Department of Otorhinolaryngology, 162181Cova da Beira Hospital Centre (CHCB), Covilhã, Portugal.
Ear Nose Throat J. 2021 Mar;100(3):177-184. doi: 10.1177/0145561319867668. Epub 2019 Sep 23.
EPOS 2012 states that investigation is needed to study a possible role for food allergy in the initiation and perpetuation of chronic rhinosinusitis with nasal polyps (CRSwNP). Our main goal was to compare serum levels of food-specific immunoglobulin G (IgG) and IgE antibodies in patients with CRSwNP and controls. A prospective case-control study with 33 patients with CRSwNP and 31 controls without CRS was carried out. Clinical data were gathered through a systematic interview and blood sample was collected. Enzyme-linked immunosorbent assay tests using OmegaDiagnostics kit with 40 food allergens for detection of specific IgG antibodies were performed and food-specific IgE antibodies were determined by immunoassay using ImmunoCAP. Immunoglobulin classes and IgG subclasses levels were also evaluated. Statistical analysis was performed using SPSS v.23. The overall sum of food IgG antibodies was significantly lower in CRSwNP compared to control group, and this difference was also observed for different specific IgG antibodies (corn, soya, grain legumes, pear and apple, berries, citric fruit). In controls, a positive correlation between IgG1 and the sum of food IgG antibodies was seen, but in CRSwNP group a negative correlation was found. In addition, a significant higher level of IgG1 and lower IgG2 and IgG3 was found among patients with CRSwNP. Levels of serum-specific IgE antibodies against multiallergen food mix (fx5) and against shrimp, strawberry, orange, rye, or egg yolk, as well as the sum of food IgE antibodies, did not differ significantly between the groups. These findings suggest that food allergy does not have an important role in CRSwNP etiopathogenesis, whether it is IgG or IgE mediated. Moreover, the observed suppression of specific IgG antibodies against food allergens, its negative correlation with IgG1 and the IgG1 switching in CRSwNP, can be related to deviated IgG responses against other targets (eg, airborne particles) and warrants future investigation.
EPOS 2012 指出，需要进行研究以探究食物过敏在慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）的起始和持续中的可能作用。我们的主要目标是比较 CRSwNP 患者和对照组患者血清中食物特异性免疫球蛋白 G（IgG）和 IgE 抗体的水平。进行了一项前瞻性病例对照研究，纳入了 33 例 CRSwNP 患者和 31 例无 CRS 的对照组患者。通过系统访谈收集临床数据，并采集血样。使用 OmegaDiagnostics 试剂盒（包含 40 种食物过敏原）进行酶联免疫吸附试验以检测特异性 IgG 抗体，使用 ImmunoCAP 进行免疫测定以确定食物特异性 IgE 抗体。还评估了免疫球蛋白类别和 IgG 亚类水平。使用 SPSS v.23 进行统计分析。与对照组相比，CRSwNP 患者的食物 IgG 抗体总量显著降低，不同特异性 IgG 抗体（玉米、大豆、谷物豆类、梨和苹果、浆果、柑橘类水果）也存在这种差异。在对照组中，IgG1 与食物 IgG 抗体总量之间存在正相关，但在 CRSwNP 组中则存在负相关。此外，在 CRSwNP 患者中发现 IgG1 水平显著升高，而 IgG2 和 IgG3 水平降低。两组之间针对多过敏原食物混合物（fx5）和虾、草莓、橙子、黑麦、或蛋黄的血清特异性 IgE 抗体水平以及食物 IgE 抗体总量均无显著差异。这些发现表明，食物过敏在 CRSwNP 的发病机制中没有重要作用，无论是 IgG 还是 IgE 介导的。此外，针对食物过敏原的特异性 IgG 抗体的观察到的抑制作用、其与 IgG1 的负相关以及 CRSwNP 中的 IgG1 转换，可能与针对其他靶标的 IgG 反应的偏离有关（例如，空气传播颗粒），值得进一步研究。
