Liu Hui, Huang Chao, Chen Shuqiang, Zheng Qing, Ye Yuhong, Ye Zhen, Lv Guorong
Department of Ultrasound, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Department of Nuclear Medicine, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
J Int Med Res. 2019 Nov;47(11):5740-5751. doi: 10.1177/0300060519874159. Epub 2019 Sep 23.
This study aimed to examine the associations between contrast-enhanced ultrasound (CEUS) imaging and synovial hypervascularity and synovitis score in a rabbit model of antigen-induced arthritis (AIA), compared with power Doppler ultrasound (PDUS).
We investigated 50 knee joints in 25 AIA rabbits (AIA group), and 10 knee joints in five sham-injected rabbits (control group). PDUS and CEUS images were evaluated at the 8 week. Ultrasound-guided synovial biopsies were targeted in the area with hypervascularity, and synovial microvessel density (MVD) was evaluated by immunohistochemical staining of CD31.
The PDUS score was significantly higher in the AIA group (2.61 ± 0.78) compared with the control group (0.50 ± 0.53). CEUS in the AIA group revealed a fast-in/slow-out pattern of contrast enhancement. MVD revealed by CD31+ vessel count and the synovitis score were significantly higher in the AIA group compared with the control group. In the AIA group, CEUS findings showed a better correlation with MVD revealed by CD31+ and synovitis score than PDUS findings.
CEUS is superior to PDUS for estimating synovial hypervascularity and hyperplasia in experimental rheumatoid arthritis.
本研究旨在与能量多普勒超声（PDUS）相比，在抗原诱导性关节炎（AIA）兔模型中，研究超声造影（CEUS）成像与滑膜血管增多及滑膜炎评分之间的关联。
我们对25只AIA兔的50个膝关节进行了研究（AIA组），并对5只假注射兔的10个膝关节进行了研究（对照组）。在第8周时评估PDUS和CEUS图像。在血管增多区域进行超声引导下的滑膜活检，并通过CD31免疫组化染色评估滑膜微血管密度（MVD）。
AIA组的PDUS评分（2.61±0.78）显著高于对照组（0.50±0.53）。AIA组的CEUS显示造影剂快速进入/缓慢退出的增强模式。与对照组相比，AIA组中通过CD31+血管计数显示的MVD和滑膜炎评分显著更高。在AIA组中，CEUS结果与通过CD31+显示的MVD和滑膜炎评分的相关性优于PDUS结果。
在评估实验性类风湿关节炎中的滑膜血管增多和增生方面，CEUS优于PDUS。
