Das Bodhisatwa, Shrirao Anil, Golberg Alexander, Berthiaume Francois, Schloss Rene, Yarmush Martin L
Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, USA.
Department of Environmental Studies, Tel Aviv University, Tel Aviv, Israel.
Bioelectricity. 2020 Jun 1;2(2):175-185. doi: 10.1089/bioe.2020.0015. Epub 2020 Jun 17.
High-powered pulsed electric fields (PEF) may be used for tissue debridement and disinfection, while lower PEF intensities may stimulate beneficial cellular responses for wound healing. We investigated the dual effects of nonuniform PEF on cellular death and stimulation. Dermal fibroblast or keratinocyte monolayers were exposed to PEF induced by two needle electrodes (2 mm apart). Voltages (100-600 V; 1 Hz; 70 micros pulse width; 90 pulses/cycle) were applied between the two electrodes. Controls consisted of similar monolayers subjected to a scratch mechanical injury. Cell growth and closure of the cell-free gap was faster in PEF-treated cell monolayers versus scratched ones. Media conditioned from cells pre-exposed to PEF, when applied to responder cells, stimulated greater proliferation than media from scratched monolayers. PEF treatment causes the release of soluble factors that promote cell growth, and thus may play a role in the accelerated healing of wounds post PEF.
高强度脉冲电场（PEF）可用于组织清创和消毒，而较低的PEF强度可能会刺激有益的细胞反应以促进伤口愈合。我们研究了非均匀PEF对细胞死亡和刺激的双重作用。将真皮成纤维细胞或角质形成细胞单层暴露于由两个针状电极（相距2毫米）诱导的PEF中。在两个电极之间施加电压（100 - 600伏；1赫兹；70微秒脉冲宽度；90个脉冲/周期）。对照组由遭受划痕机械损伤的相似单层细胞组成。与划痕处理的细胞单层相比，PEF处理的细胞单层的细胞生长和无细胞间隙的闭合更快。预先暴露于PEF的细胞所产生的条件培养基，当应用于反应细胞时，比划痕单层细胞的培养基刺激更大的增殖。PEF处理会导致促进细胞生长的可溶性因子的释放，因此可能在PEF后伤口的加速愈合中发挥作用。
