Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, School of Life Sciences, Northwest University, Xi'an, 710069, China.
Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, School of Life Sciences, Beijing Normal University, Beijing, 100875, China.
Biochem Biophys Res Commun. 2019 Nov 19;519(4):783-789. doi: 10.1016/j.bbrc.2019.09.047. Epub 2019 Sep 21.
Nuclear migration in Arabidopsis root hairs is bidirectional and relies on actin filaments. However, how actin filaments regulate the bidirectional movement of nuclei remains unclear. Here, we discovered that nuclei migrate forward and backward according to the developmental stage of the hair cells. In addition, the migration direction of nuclei was not constant but reversed occasionally, accompanied by nuclear shape changes. Confocal microscopic analysis revealed that perinuclear actin bundles were closely related to the migration and shape of hair cell nuclei. Pharmacological studies showed that SMIFH2, an inhibitor of the actin nucleator-formin, inhibited nuclear backward migration probably by impairing the perinuclear actin filaments. These data indicate that nuclear migration in hair cells is likely motivated by the competition of mechanical forces acting on the nucleus. Furthermore, the perinuclear actin filaments are closely related to the migration direction of hair cell nuclei.
拟南芥根毛中的核迁移是双向的，依赖于肌动蛋白丝。然而，肌动蛋白丝如何调节核的双向运动仍不清楚。在这里，我们发现核根据毛细胞的发育阶段向前和向后迁移。此外，核的迁移方向不是固定的，而是偶尔会发生反转，伴随着核形状的变化。共聚焦显微镜分析显示，核周肌动蛋白束与毛细胞核的迁移和形状密切相关。药理学研究表明，肌动蛋白成核因子formin 的抑制剂 SMIFH2 可能通过破坏核周肌动蛋白丝来抑制核的向后迁移。这些数据表明，毛细胞中的核迁移可能是由作用于核的机械力竞争驱动的。此外，核周肌动蛋白丝与毛细胞核的迁移方向密切相关。
