Mata Clara, Longmire Ellen, McKenna David, Glass Katie, Hubel Allison
Aerospace Engineering and Mechanics, University of Minnesota, 110 Union Street SE, Minneapolis, MN 55455, USA.
Clinical Cell Therapy Laboratory, University of Minnesota, 420 Delaware Street, Minneapolis, MN 55455, USA.
Microfluid Nanofluidics. 2010 Apr;8(4):457-465. Epub 2009 Jul 24.
The motion of cells in a two-stream microfluidic device designed to extract cryoprotective agents from cell suspensions was tested under a range of conditions. Jurkat cells (lymphoblasts) in a 10% dimethylsulfoxide solution were driven in parallel with phosphate-buffered saline solution wash streams through single rectangular channel sections and multiple sections in series. The influence of cell-stream flow rate and cell volume fraction (CVF) on cell viability and recovery were examined. The channel depth was 500 lm, and average cell stream velocity within the channels was varied from 3.6 to8.5 mm/s corresponding with cell stream Reynolds numbers of 2.6-6.0. Cell viability measured at device outlets was high for all cases examined indicating no significant cell damage within the device. Downstream of a single stage, cell recoveries measured 90-100% for average cell stream velocities ≥6 mm/s and for CVFs up to 20%. Cell recovery downstream of multistage devices also measured 90-100% after a critical device population time. This time was found to be five times the average cell residence time within the device. The measured recovery values were significantly larger than those typically obtained using conventional cell washing methods.
在一系列条件下，对一种用于从细胞悬液中提取冷冻保护剂的双流微流控装置中的细胞运动进行了测试。将处于10%二甲基亚砜溶液中的Jurkat细胞（成淋巴细胞）与磷酸盐缓冲盐溶液冲洗流平行驱动，使其通过单个矩形通道段和多个串联的通道段。研究了细胞流流速和细胞体积分数（CVF）对细胞活力和回收率的影响。通道深度为500μm，通道内平均细胞流速度在3.6至8.5mm/s之间变化，对应的细胞流雷诺数为2.6 - 6.0。在所研究的所有情况下，在装置出口处测得的细胞活力都很高，这表明装置内没有明显的细胞损伤。在单级下游，对于平均细胞流速度≥6mm/s且CVF高达20%的情况，细胞回收率为90 - 100%。在经过关键的装置停留时间后，多级装置下游的细胞回收率也为90 - 100%。发现这段时间是细胞在装置内平均停留时间的五倍。测得的回收率值明显大于使用传统细胞洗涤方法通常获得的值。
