Koh Byumseok, Kim Kwang Rok
Bio and Drug Discovery Division, Korea Research Institute of Chemical Technology, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, Republic of Korea.
ACS Omega. 2019 Aug 30;4(12):15134-15138. doi: 10.1021/acsomega.9b02021. eCollection 2019 Sep 17.
Monitoring of long-term stability of proteins on paper-based membranes is important as it is directly related to paper-based sensor fabrication. By using a simple piezo printhead inkjet printer, recombinant proteins and antibodies were printed on paper-based membranes to test their stability and sensitivity under varying lengths of storage and temperature conditions. Our data show that a printed IgG-HRP antibody on simple printing paper maintains >50% functionality up to ∼2 months under 4 and -20 °C storage. Antibodies printed on polyvinylidene difluoride (PVDF) and nitrocellulose showed 5.3 and 9.7% decreases, respectively, in initial signal intensities compared to printing paper. Prostate-specific membrane antigen and tumor necrosis factor alpha recombinant proteins printed on paper-based membranes can be detected by antibodies, and antibody signal intensities can be detected up to 28 days after storage at 4 and -20 °C when printed on PVDF membrane or printing paper. These data suggest that printed proteins on simple printing paper and PVDF membrane can maintain their functionality up to few months when stored at 4 °C or lower and can be potentially applied in paper-based sensor development.
监测蛋白质在纸质膜上的长期稳定性非常重要，因为这与基于纸张的传感器制造直接相关。通过使用简单的压电打印头喷墨打印机，将重组蛋白和抗体打印在纸质膜上，以测试它们在不同储存时间和温度条件下的稳定性和灵敏度。我们的数据表明，在普通打印纸上打印的IgG-HRP抗体在4℃和-20℃储存条件下，长达约2个月仍保持>50%的功能。与打印纸相比，打印在聚偏二氟乙烯（PVDF）和硝酸纤维素上的抗体初始信号强度分别下降了5.3%和9.7%。打印在纸质膜上的前列腺特异性膜抗原和肿瘤坏死因子α重组蛋白可以被抗体检测到，当打印在PVDF膜或打印纸上时，在4℃和-20℃储存28天后仍可检测到抗体信号强度。这些数据表明，普通打印纸和PVDF膜上打印的蛋白质在4℃或更低温度下储存时，其功能可以维持数月，并有可能应用于基于纸张的传感器开发。
