急性髓系白血病患者中SALL4基因和BMI-1基因表达的研究
The Study of SALL4 Gene and BMI-1 Gene Expression in Acute Myeloid Leukemia Patients.
作者信息
Swelem Rania Shafik, Elneely Dalia Abdelmoety, Shehata Ahmed Abdel Rahman
机构信息
Department of Clinical and Chemical Pathology, Faculty of Medicine, Egypt.
Department ofInternal Medicine (Hematology), Faculty of Medicine, Alexandria University, Egypt.
出版信息
Lab Med. 2020 May 6;51(3):265-270. doi: 10.1093/labmed/lmz056.
BACKGROUND
In acute myeloid leukemia (AML), many genes have been studied as prognostic markers. SALL4 is expressed constitutively in human leukemia cell lines and primary AML cells. BMI-1 is expressed highly in purified hematopoietic stem cells (HSCs), and its expression declines with differentiation.
OBJECTIVE
To study the expression levels of SALL4 and BMI-1 and their clinical significance in patients with AML.
METHODS
The study was performed with 60 patients newly diagnosed with AML and 50 control individuals. SALL4 and BMI-1 expression detection were performed using real-time polymerase chain reaction (PCR).
RESULTS
The expression of SALL4 and BMI-1 was significantly higher in cases of AML and showed a strong association with failure to achieve complete remission (CR) or with relapse (P = .02, P = .03, respectively). In multivariate analysis, these genes were the most powerful independent predictors of poor prognosis (P = .01 for SALL4, P = .02 for BMI-1).
CONCLUSION
SALL4 and BMI-1 are significant prognostic factors in AML and could be strong targets for novel types of therapy.
背景
在急性髓系白血病（AML）中，许多基因已被作为预后标志物进行研究。SALL4在人白血病细胞系和原发性AML细胞中持续表达。BMI-1在纯化的造血干细胞（HSC）中高表达，且其表达随分化而下降。
目的
研究SALL4和BMI-1在AML患者中的表达水平及其临床意义。
方法
对60例新诊断的AML患者和50例对照个体进行研究。使用实时聚合酶链反应（PCR）检测SALL4和BMI-1的表达。
结果
SALL4和BMI-1在AML病例中的表达显著更高，且与未达到完全缓解（CR）或复发密切相关（分别为P = 0.02，P = 0.03）。在多变量分析中，这些基因是预后不良的最有力独立预测因子（SALL4为P = 0.01，BMI-1为P = 0.02）。
结论
SALL4和BMI-1是AML的重要预后因素，可能成为新型治疗的有力靶点。
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