Steinkampf M P, Mendelson C R, Simpson E R
Department of Obstetrics and Gynecology, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Health Science Center Dallas 75235-9051.
Mol Endocrinol. 1987 Jul;1(7):465-71. doi: 10.1210/mend-1-7-465.
The effects of FSH to increase the activity of aromatase, as well as the synthesis of the components of the aromatase enzyme complex, have been studied in human ovarian granulosa cells obtained from women undergoing oocyte retrieval. FSH increased aromatase activity, as well as the synthesis of aromatase cytochrome P-450 (P-450AROM) in a time-dependent fashion, whereas in the absence of FSH, both activity and synthesis declined with duration of culture. The effect of FSH was mimicked by forskolin, an activator of adenylate cyclase. FSH also increased the synthesis of NADPH-cytochrome P-450 reductase, but to a relatively modest extent. The levels of hybridizable mRNA species encoding cytochrome P-450AROM of lengths 3.0, 2.4, and 1.6 kilobases were also increased with FSH treatment. It is concluded that the regulation of aromatase activity by FSH in human granulosa cells is mediated primarily by changes in the synthesis of cytochrome P-450AROM, that this action of FSH is mediated by cAMP, and that the changes in cytochrome P-450AROM synthesis are the consequences of changes in the levels of mRNA encoding this enzyme.
在从接受取卵手术的女性获取的人卵巢颗粒细胞中，研究了促卵泡激素（FSH）增加芳香化酶活性以及芳香化酶酶复合物各组分合成的作用。FSH以时间依赖性方式增加芳香化酶活性以及芳香化酶细胞色素P - 450（P - 450AROM）的合成，而在无FSH时，随着培养时间延长，活性和合成均下降。FSH的作用可被腺苷酸环化酶激活剂福斯可林模拟。FSH还增加了NADPH - 细胞色素P - 450还原酶的合成，但程度相对较小。经FSH处理后，编码长度为3.0、2.4和1.6千碱基的细胞色素P - 450AROM的可杂交mRNA种类水平也增加。得出的结论是，FSH对人颗粒细胞中芳香化酶活性的调节主要通过细胞色素P - 450AROM合成的变化介导，FSH的这一作用由环磷酸腺苷（cAMP）介导，并且细胞色素P - 450AROM合成的变化是编码该酶的mRNA水平变化的结果。
