Steinkampf M P, Mendelson C R, Simpson E R
Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas 75235-9051.
Mol Cell Endocrinol. 1988 Sep;59(1-2):93-9. doi: 10.1016/0303-7207(88)90199-2.
The effects of growth factors to regulate the activity of aromatase, as well as the synthesis of aromatase cytochrome P-450 (P-450AROM) have been studied in human ovarian granulosa cells obtained from women undergoing oocyte retrieval. Insulin-like growth factor I (IGF-I) increased aromatase activity as well as the synthesis of P-450AROM, in a concentration-dependent fashion. The levels of hybridizable mRNA species encoding cytochrome P-450AROM were also increased with IGF-I treatment. By contrast, epidermal growth factor (EGF) had no effect on these parameters when added alone, but markedly inhibited the action of follicle-stimulating hormone (FSH) to stimulate aromatase activity, and the synthesis of cytochrome P-450AROM, as well as its ability to increase the levels of mRNA encoding the enzyme. It is concluded that these growth factors have opposite effects on aromatase activity, and that these actions reflect, in part, changes in the synthesis of cytochrome P-450AROM, which in turn are the consequence of changes in the levels of mRNA encoding this enzyme.
在从接受取卵手术的女性身上获取的人卵巢颗粒细胞中，研究了生长因子对芳香化酶活性以及芳香化酶细胞色素P - 450（P - 450AROM）合成的影响。胰岛素样生长因子I（IGF - I）以浓度依赖的方式增加了芳香化酶活性以及P - 450AROM的合成。用IGF - I处理后，编码细胞色素P - 450AROM的可杂交mRNA种类水平也有所增加。相比之下，单独添加表皮生长因子（EGF）时对这些参数没有影响，但能显著抑制促卵泡激素（FSH）刺激芳香化酶活性、细胞色素P - 450AROM合成以及其增加该酶编码mRNA水平的能力。得出的结论是，这些生长因子对芳香化酶活性有相反的作用，并且这些作用部分反映了细胞色素P - 450AROM合成的变化，而这又是该酶编码mRNA水平变化的结果。
