果蝇幼虫大脑中神经干细胞的活细胞成像
Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain.
作者信息
Miszczak Karolina, Egger Boris
机构信息
Department of Biology, University of Fribourg, Fribourg, Switzerland.
出版信息
Methods Mol Biol. 2020;2047:153-160. doi: 10.1007/978-1-4939-9732-9_9.
Live cell imaging gives valuable insights into the dynamic biological processes within and between cells. An important aspect of live cell imaging is to keep the cells under best physiological condition and to prevent abnormal cellular behavior, which might be caused by phototoxicity during microscopy. In this chapter we describe a protocol to visualize division patterns of neural stem cells in live whole mount brains of Drosophila larvae. We also present a newly developed live cell chamber that allows us to control the environmental air during live cell imaging. The protocol can be adapted to look at a wide range of cellular and tissue behavior in the Drosophila model system.
活细胞成像为深入了解细胞内和细胞间的动态生物学过程提供了有价值的见解。活细胞成像的一个重要方面是使细胞保持在最佳生理条件下，并防止可能由显微镜检查期间的光毒性引起的异常细胞行为。在本章中，我们描述了一种在果蝇幼虫的活整装大脑中可视化神经干细胞分裂模式的方案。我们还展示了一种新开发的活细胞室，它使我们能够在活细胞成像期间控制环境空气。该方案可适用于观察果蝇模型系统中广泛的细胞和组织行为。
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