Weng Mo, Komori Hideyuki, Lee Cheng-Yu
Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA.
Methods Mol Biol. 2012;879:39-46. doi: 10.1007/978-1-61779-815-3_3.
The balance between self-renewal and differentiation must be tightly regulated in somatic stem cells to ensure proper tissue generation and to prevent tumorlike overgrowth. A Drosophila larval brain lobe consists of the central brain and the optic lobe and possesses three well-defined neural stem cell lineages that generate differentiated cells in a highly reproducible pattern. Unambiguous identification of various cell types in these stem cell lineages is pivotal for studying the regulation of neural stem cells and progenitor cells at a single-cell resolution. This chapter will describe the methodology for collection and processing of larval brains for examination by fluorescence confocal microscopy.
在体细胞干细胞中，自我更新与分化之间的平衡必须受到严格调控，以确保组织正常生成并防止肿瘤样过度生长。果蝇幼虫脑叶由中枢脑和视叶组成，并拥有三个明确的神经干细胞谱系，这些谱系以高度可重复的模式生成分化细胞。在单细胞分辨率下明确识别这些干细胞谱系中的各种细胞类型，对于研究神经干细胞和祖细胞的调控至关重要。本章将描述用于收集和处理幼虫脑以便通过荧光共聚焦显微镜进行检查的方法。
