Streit Andrea, Stern Claudio D
Faculty of Dental, Oral and Craniofacial Sciences, Centre for Craniofacial and Regenerative Biology, King's College London, London, UK.
Department of Cell and Developmental Biology, University College London, London, UK.
Methods Mol Biol. 2020;2047:457-473. doi: 10.1007/978-1-4939-9732-9_26.
Tissue transplantation is an important approach in developmental neurobiology to determine cell fate, to uncover inductive interactions required for tissue specification and patterning as well as to establish tissue competence and commitment. Combined with state-of-the-art molecular approaches, transplantation assays have been instrumental for the discovery of gene regulatory networks controlling cell fate choices and how such networks change over time. Avian species are among the favorite model systems for these approaches because of their accessibility and relatively large size. Here we describe two culture techniques used to generate quail-chick chimeras at different embryonic stages and methods to distinguish graft and donor tissue.
组织移植是发育神经生物学中一种重要的方法，用于确定细胞命运、揭示组织特化和模式形成所需的诱导相互作用，以及建立组织能力和定向分化。移植实验与最先进的分子方法相结合，有助于发现控制细胞命运选择的基因调控网络，以及这些网络如何随时间变化。由于易于操作且体型相对较大，鸟类是这些方法最常用的模型系统之一。在这里，我们描述了两种用于在不同胚胎阶段生成鹌鹑 - 鸡嵌合体的培养技术，以及区分移植组织和供体组织的方法。
