Le Douarin N M, Tan K, Hallonet M, Kinutani M
Institut d'Embryologie Cellulaire et Moléculaire du CNRS et du Collège de France, Nogent-sur-Marne.
Kaibogaku Zasshi. 1993 Apr;68(2):152-61.
Avian embryonic neural chimeras are constructed by substituting defined areas of the neural epithelium of a chick embryo by their exact counterpart obtained from a quail embryo at the same developmental stage. The experiment can also be performed using the quail as a host and the chick as a graft donor. The stages elected are either the late neurula (0 to 3 somites) or the stage after cephalic vesicles formation (10 to 14 somites) but in all cases before the onset of vascularization of the neural primordium. Quail and chick territories can be recognized in the chimeras any time after the graft owing to the particular structure of the quail nucleus or by means of species-specific antibodies. Quail-chick chimeras have been instrumental in the study of the ontogeny of the medulla oblongata and of the cerebellum. The complex morphogenetic movements and cell migrations which occur during the development of this part of the brain have been worked out by this method. The main results obtained are described in the article.
鸟类胚胎神经嵌合体是通过用处于相同发育阶段的鹌鹑胚胎的精确对应部位，替换鸡胚胎神经上皮的特定区域构建而成的。该实验也可以用鹌鹑作为宿主、鸡作为移植供体来进行。选取的阶段要么是神经胚后期（0至3体节），要么是头泡形成后的阶段（10至14体节），但在所有情况下均是在神经原基血管化开始之前。由于鹌鹑细胞核的特殊结构或借助物种特异性抗体，移植后任何时候都能在嵌合体中识别出鹌鹑和鸡的区域。鹌鹑 - 鸡嵌合体在延髓和小脑个体发育的研究中发挥了重要作用。通过这种方法已经明确了大脑这一部分发育过程中发生的复杂形态发生运动和细胞迁移。文章中描述了所获得的主要结果。
