Akbari Morteza, Shanehbandi Dariush, Asadi Milad, Shomali Navid, Faraji Afsaneh, Khaze Vahid, Pakdel Abbas, Mokhtarzadeh Ahad, Ebrahimi Ali Asghar, Shabani Aliakbar, Bardaran Behzad
Department of Biotechnology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Iran J Immunol. 2019 Sep;16(3):246-257. doi: 10.22034/IJI.2019.80275.
Colorectal cancer (CRC) is attributed as one of the most common malignancies worldwide. CD133 molecule, as a pentaspan transmembrane glycoprotein, confers stem cell-related characteristics, including self-renewal and multi-directional differentiation capability. CD133 plays important roles in the progression of CRC by conferring apoptotic resistance and migration ability.
To investigate the anti-apoptotic and anti-angiogenic effect of CD-133 targeted siRNA in a colorectal cancer cell line.
In this study, CD133-targeted siRNA transfection was conducted into HT-29 cells. MTT assay was employed to evaluate the cytotoxic effects of transfection on the cells. Flow cytometry was used to evaluate the apoptosis rate. The mRNA expression of apoptosis and metastasis related genes were assessed by quantitative Real-Time PCR (qRT-PCR). Wound healing assay was used to assess the migration potency of the infected cells.
Expression of CD133 was significantly downregulated after transfection of CD133-specific siRNA. Moreover, the rate of apoptosis was significantly increased after transfection. The migration potential of cells was diminished after transfection. siRNA delivery resulted in the modulation of expression of apoptosis and metastasis-related genes.
siRNA mediated targeting of CD133 could be considered as a promising approach to treat CRC through suppressing the cancerous behavior of tumor cells.
结直肠癌（CRC）被认为是全球最常见的恶性肿瘤之一。CD133分子作为一种五跨膜糖蛋白，赋予干细胞相关特性，包括自我更新和多向分化能力。CD133通过赋予抗凋亡和迁移能力在CRC进展中发挥重要作用。
研究CD-133靶向小干扰RNA（siRNA）对结直肠癌细胞系的抗凋亡和抗血管生成作用。
本研究将CD133靶向siRNA转染至HT-29细胞。采用MTT法评估转染对细胞的细胞毒性作用。流式细胞术用于评估凋亡率。通过定量实时聚合酶链反应（qRT-PCR）评估凋亡和转移相关基因的mRNA表达。采用划痕试验评估感染细胞的迁移能力。
转染CD133特异性siRNA后，CD133表达显著下调。此外，转染后凋亡率显著增加。转染后细胞的迁移能力减弱。siRNA传递导致凋亡和转移相关基因表达的调节。
siRNA介导的CD133靶向作用可被视为一种通过抑制肿瘤细胞的癌性行为来治疗CRC的有前景的方法。
