Amrani-Allalou Hanane, Boulekbache-Makhlouf Lila, Mapelli-Brahm Paula, Sait Sabrina, Tenore Gian Carlo, Benmeziane Akila, Kadri Nabil, Madani Khodir, Jesús Meléndez Martínez Antonio
Biologie Physico-Chimique, Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, Algérie.
Food sciences, Université A.Mira de Bejaia, Bejaia 06000, Algeria.
J Complement Integr Med. 2019 Sep 17;17(1):/j/jcim.2019.17.issue-1/jcim-2017-0081/jcim-2017-0081.xml. doi: 10.1515/jcim-2017-0081.
Background Plant and medicinal herbs are important sources of bioactive compounds and minerals that can play a role in preventing various diseases and they are considered a factor indispensable for the proper functioning of the human body. Methods We investigated the content of carotenoids and chlorophylls of leaves from Pallenis spinosa (P. spinosa), as well as their antioxidant activity and mineral composition then, we optimized the solvent extraction for the recovery of total carotenoids and chlorophylls using spectrophotometric method. Finally, we tested the antioxidant activity of the optimized extract by three assays (DPPH, ABTS and FRAP) and we determined the mineral composition by Emission Spectrometer Induced Couple Plasma (ICP). Results Carotenoid (CART), chlorophylls (CHLa + b), chlorophyll a (CHLA), chlorophyll b (CHLB) contents were about 36.337 ± 0.312; 347.769 ± 6.326; 224.286 ± 5.601; 123.483 ± 1.339 mg/100 g dw, respectively. We revealed an interesting antioxidant capacity by the tested extract (DPPH: 127.522 ± 1.406 mmol ET/Kgdw, ABTS: 104.827 ± 1.222 mmol ET/Kgdw and FRAP 71.89 ± 0.495 ± 0.994 mmol ET/Kgdw). Carotenoids and chlorophylls content correlate positively with the antioxidant activity of P. spinosa leaves extract (r=0.646-0.986). Eight minerals have been detected (Mg, Ca, P, Fe, Mn, Zn, Cu and Cr), Mg and Ca being the predominant ones (6479.32 ± 48.33 and 3851.88 ± 130.63 mg/Kg, respectively). Conclusions These results have shown that P. spinosa leaves are a good source of carotenoids and chlorophylls with a potent antioxidant potential with high amount of minerals.
背景 植物和药草是生物活性化合物和矿物质的重要来源，它们在预防各种疾病中发挥作用，并且被认为是人体正常运转不可或缺的因素。方法 我们研究了刺苞菜（Pallenis spinosa，P. spinosa）叶片中类胡萝卜素和叶绿素的含量、它们的抗氧化活性和矿物质组成，然后，我们使用分光光度法优化了用于回收总类胡萝卜素和叶绿素的溶剂萃取方法。最后，我们通过三种测定法（DPPH、ABTS和FRAP）测试了优化提取物的抗氧化活性，并通过电感耦合等离子体发射光谱仪（ICP）测定了矿物质组成。结果 类胡萝卜素（CART）、叶绿素（CHLa + b）、叶绿素a（CHLA）、叶绿素b（CHLB）的含量分别约为36.337±0.312；347.769±6.326；224.286±5.601；123.483±1.339 mg/100 g干重。我们发现测试提取物具有有趣的抗氧化能力（DPPH：127.522±1.406 mmol ET/Kgdw，ABTS：104.827±1.222 mmol ET/Kgdw，FRAP：71.89±0.495±0.994 mmol ET/Kgdw）。类胡萝卜素和叶绿素含量与刺苞菜叶片提取物的抗氧化活性呈正相关（r = 0.646 - 0.986）。已检测到八种矿物质（Mg、Ca、P、Fe、Mn、Zn、Cu和Cr），Mg和Ca含量最高（分别为6479.32±48.33和3851.88±130.63 mg/Kg）。结论 这些结果表明，刺苞菜叶片是类胡萝卜素和叶绿素的良好来源，具有强大的抗氧化潜力和大量的矿物质。
