Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
Food Colour & Quality Lab., Area of Nutrition & Food Science, Universidad de Sevilla, Seville, Spain.
Food Res Int. 2018 Sep;111:391-398. doi: 10.1016/j.foodres.2018.05.044. Epub 2018 May 21.
The carotenoid and mineral levels as well as the in vitro antioxidant capacity, using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, of waste from cantaloupe was assessed. Then the matrix was subjected to ultrasound-assisted extraction (UAE) and response surface methodology (RSM) was used for the optimization of the extraction of carotenoids. The effect of the extraction procedure on the microstructure of the powder was assessed by scanning electron microscopy (SEM) analysis. The major carotenoids identified were lutein (63.24 ± 0.73 μg βCE/g dw) and β-carotene (56.43 ± 0.11 μg βCE/g dw). Several mineral elements (K, Na, P, Mg, Ca, Fe, Cu, Mn and Zn) were identified, potassium being the major one.The extract exhibited in vitro antioxidant activity (IC = 7.33 ± 0.22 μg/mL). The RSM results showed that an amplitude of 100%, extraction time of 10 min, hexane percentage of 80% in hexane/acetone solvent, and solvent-to-solid ratio of 55 mL/g were the optimal conditions for the extraction of carotenoids. Under these conditions, the carotenoid content of the extract was 124.61 ± 3.82 μg/g.The microscopic analysis revealed the effectiveness of the ultrasound treatment that results in noticeable physical changes, like microscopic perforations and breakages.
评估了废弃哈密瓜中的类胡萝卜素和矿物质水平，以及使用 1,1-二苯基-2-苦基肼（DPPH）自由基清除测定法的体外抗氧化能力。然后，将基质进行超声辅助提取（UAE），并使用响应面法（RSM）优化类胡萝卜素的提取。通过扫描电子显微镜（SEM）分析评估了提取程序对粉末微观结构的影响。鉴定出的主要类胡萝卜素为叶黄素（63.24±0.73μg βCE/g dw）和β-胡萝卜素（56.43±0.11μg βCE/g dw）。鉴定出几种矿物质元素（K、Na、P、Mg、Ca、Fe、Cu、Mn 和 Zn），其中钾含量最高。提取物表现出体外抗氧化活性（IC=7.33±0.22μg/mL）。RSM 结果表明，振幅为 100%、提取时间为 10 分钟、正己烷/丙酮溶剂中正己烷的百分比为 80%、溶剂与固体的比例为 55 mL/g 是提取类胡萝卜素的最佳条件。在此条件下，提取物中的类胡萝卜素含量为 124.61±3.82μg/g。微观分析显示了超声处理的有效性，导致明显的物理变化，如微观穿孔和破裂。
